Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
| Autor: | Susan Clarke, Nicholas Dunne, Eoin Cunningham, Fraser Buchanan, Seong Ying Choi, Melanie McKechnie, Gavin Walker, George A. Burke |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Scaffold
Materials science Cellular differentiation Guinea Pigs Biomedical Engineering Biophysics Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Tissue engineering Biomimetic Materials Osteogenesis Materials Testing medicine Animals Humans SDG 14 - Life Below Water Porosity Cytotoxicity Cells Cultured Polyurethane Osteoblasts Tissue Engineering Tissue Scaffolds biology Cell Differentiation Osteoblast Biomaterials Synthesis and Characterization 021001 nanoscience & nanotechnology biology.organism_classification Porifera 0104 chemical sciences Sponge Durapatite medicine.anatomical_structure chemistry Chemical Engineering(all) 0210 nano-technology Biomedical engineering |
| Zdroj: | Clarke, S A, Choi, S Y, McKechnie, M, Burke, G, Dunne, N, Walker, G, Cunningham, E & Buchanan, F 2016, ' Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges ', Journal of Materials Science: Materials in Medicine, vol. 27, no. 2, pp. 1-11 . https://doi.org/10.1007/s10856-015-5630-0 Journal of Materials Science. Materials in Medicine |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-015-5630-0 |
| Popis: | Bone tissue engineering may provide an alternative to autograft, however scaffold optimisation is required to maximize bone ingrowth. In designing scaffolds, pore architecture is important and there is evidence that cells prefer a degree of non-uniformity. The aim of this study was to compare scaffolds derived from a natural porous marine sponge (Spongia agaricina) with unique architecture to those derived from a synthetic polyurethane foam. Hydroxyapatite scaffolds of 1 cm3 were prepared via ceramic infiltration of a marine sponge and a polyurethane (PU) foam. Human foetal osteoblasts (hFOB) were seeded at 1x105 cells/scaffold for up to 14 days. Cytotoxicity, cell number, morphology and differentiation were investigated. PU-derived scaffolds had 84-91% porosity and 99.99% pore interconnectivity. In comparison marine sponge-derived scaffolds had 56-61% porosity and 99.9% pore interconnectivity. hFOB studies showed that a greater number of cells were found on marine sponge-derived scaffolds at than on the PU scaffold but there was no significant difference in cell differentiation. X-ray diffraction (XRD) and inductively coupled plasma mass spectrometry (ICP-MS) showed that Si ions were released from the marine-derived scaffold. In summary, three dimensional porous constructs have been manufactured that support cell attachment, proliferation and differentiation but significantly more cells were seen on marine-derived scaffolds. This could be due both to the chemistry and pore architecture of the scaffolds with an additional biological stimulus from presence of Si ions. Further in vivo tests in orthotopic models are required but this marine-derived scaffold shows promise for applications in bone tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink