Autophagy Attenuates Angiotensin II-Induced Pulmonary Fibrosis by Inhibiting Redox Imbalance-Mediated NOD-Like Receptor Family Pyrin Domain Containing 3 Inflammasome Activation
Autor: | Xu Li, Na-Na Sun, Qianjie Yang, Miaoxia Pan, Yue Zhang, Wei Li, Yan Chen, Zemao Zheng, Bo-Jun Zheng, Ying Meng |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Physiology Inflammasomes Pulmonary Fibrosis Clinical Biochemistry Biochemistry Pyrin domain 03 medical and health sciences Mice Pulmonary fibrosis NLR Family Pyrin Domain-Containing 3 Protein medicine Autophagy Animals Humans Rats Wistar Receptor Molecular Biology Cells Cultured General Environmental Science chemistry.chemical_classification Reactive oxygen species integumentary system 030102 biochemistry & molecular biology Chemistry Angiotensin II NOD-like receptor Inflammasome Cell Biology medicine.disease Cell biology Rats Mice Inbred C57BL 030104 developmental biology General Earth and Planetary Sciences Oxidation-Reduction medicine.drug |
Zdroj: | Antioxidantsredox signaling. 30(4) |
ISSN: | 1557-7716 |
Popis: | The NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome, which is activated by reactive oxygen species (ROS) and repressed by autophagy, has been identified as a novel agent of pulmonary fibrosis. Angiotensin II (AngII), the bioactive pro-oxidant in the renin-angiotensin system, aggravates lung fibrosis. However, the effect of AngII on NLRP3 inflammasome and autophagy in lung fibrosis remains unknown. This study investigates the potential link between AngII-induced autophagy in the regulation of NLRP3 inflammasome/IL-1β axis in lung fibrosis.In vivo, autophagy and the NLRP3 inflammasome were activated in fibrotic patients and positively correlated with oxidation. Treatment with rapamycin promoted autophagy but inhibited oxidation, NLRP3 inflammasome, and lung fibrosis after bleomycin (BLM) infusion. The autophagy inhibitor 3-methyladenine reduced BLM-induced lung fibrosis and concurrently facilitated NLRP3 inflammasome activation and oxidation in fibroblasts. In vitro, AngII promoted intercellular ROS, hydrogen peroxide, and NADPH oxidase 4 (NOX4) protein levels and reduced the glutathione concentration, thereby leading to NLRP3 inflammasome activation and consequent collagen synthesis. AngII induced autophagy, while VAS2870, NOX4, small-interfering RNA (siRNA), and compound C eliminated AngII-induced LC3B augmentation. Moreover, blocking autophagy with bafilomycin A1 or LC3B siRNA resulted in oxidant accumulation, NLRP3 inflammasome hyperactivation, and collagen deposition. Finally, AngII induced P62/SQSTM1, targeting ubiquitinated apoptosis-associated speck-like protein containing a CARD for degradation, thereby contributing to NLRP3 inflammasome inactivation. Innovation and Conclusion: Autophagy attenuates pulmonary fibrosis by regulating NLRP3 inflammasome activation induced by AngII-mediated ROS via redox balance modulation. |
Databáze: | OpenAIRE |
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