Synthesis and Characterization of Cationic Hydrogels from Thiolated Copolymers for Independent Manipulation of Mechanical and Chemical Properties of Cell Substrates

Autor: Florian Pätzold, Nils Stamm, Dominic Kamps, Maria Specht, Patrick Bolduan, Leif Dehmelt, Ralf Weberskirch
Rok vydání: 2022
Předmět:
Zdroj: Macromolecular Bioscience. 22:2100453
ISSN: 1616-5195
1616-5187
Popis: Cells sense both mechanical and chemical properties in their environment and respond to these inputs with altered phenotypes. Precise and selective experimental manipulations of these environmental cues require biocompatible synthetic materials, for which multiple properties can be fine-tuned independently from each other. For example, cells typically show critical thresholds for cell adhesion as a function of substrate parameters such as stiffness and the degree of functionalization. However, the choice of tailor-made, defined materials to produce such cell adhesion substrates is still very limited. Here, a platform of synthetic hydrogels based on well-defined thiolated copolymers is presented. Therefore, four disulfide crosslinked hydrogels of different composition by free radical polymerization are prepared. After cleavage with dithiothreitol, four soluble copolymers P1-P4 with 0-96% cationic monomer content are obtained. P1 and P4 are then combined with PEGDA
Databáze: OpenAIRE
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