Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review
| Autor: | Benner Geraldo Alves, Eduardo L. Gastal, G. D. A. Gastal, José Ricardo de Figueiredo, K.A. Alves, F.L.N. Aguiar |
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| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Population Ovary Biology Andrology 03 medical and health sciences 0302 clinical medicine Estrus Ovarian Follicle Food Animals Follicular phase medicine Animals Horses Small Animals education Estrous cycle education.field_of_study 030219 obstetrics & reproductive medicine Equine Ovarian tissue Critical factors 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science In vitro medicine.anatomical_structure Oocytes Tissue and Organ Harvesting Female Animal Science and Zoology Folliculogenesis |
| Zdroj: | Theriogenology. 156:283-295 |
| ISSN: | 0093-691X |
| Popis: | The mammalian ovary is responsible for essential stages of folliculogenesis and hormonal production, regulating the female physiological functions during the menstrual/estrous cycles. The mare has been considered an attractive model for comparative studies due to the striking similarities shared with women regarding in vivo and in vitro folliculogenesis. The ovarian follicular population in horses contains a large number of oocytes enclosed in preantral follicles that are yet to be explored. Therefore, the in vitro manipulation of equine preantral follicles aims to avoid the process of atresia and promote the development of follicles with competent oocytes. In this regard, after ovarian tissue harvesting, the use of appropriate processing techniques, as well as suitable approaches to evaluating equine preantral follicles and ovarian tissue, are necessary. Although high-quality equine ovarian tissue can be obtained from several sources, some critical aspects, such as the age of the animals, ovarian cyclicity, reproductive phase, and the types of ovarian structures, should be considered. Therefore, this review will focus on providing an update on the most current advances concerning the critical factors able to influence equine preantral follicle quality and quantity. Also, the in vivo strategies used to harvest equine ovarian tissue, the approaches to manipulating ovarian tissue post-harvesting, the techniques for processing ovarian tissue, and the classical approaches used to evaluate preantral follicles will be discussed. |
| Databáze: | OpenAIRE |
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