Toxicological study of sodium selenite on fetal development and DNA fragmentation in liver cells of pregnant rats
| Autor: | Mona A. M. Helal |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Liver cytology Endocrinology Diabetes and Metabolism Sodium Clinical Biochemistry chemistry.chemical_element DNA Fragmentation medicine.disease_cause Biochemistry Inorganic Chemistry Lipid peroxidation Fetal Development chemistry.chemical_compound Sodium Selenite Pregnancy Internal medicine medicine Animals Rats Wistar Fetus Biochemistry (medical) General Medicine DNA Rats Endocrinology chemistry Liver Toxicity DNA fragmentation Female Selenium Oxidative stress |
| Zdroj: | Biological trace element research. 140(1) |
| ISSN: | 1559-0720 |
| Popis: | The present study was carried out to evaluate the effects of sodium selenite on fetal development and DNA in liver of rats. Pregnant rats were divided into three groups: control group, group treated orally with 5 µg Se/kg body wt. and group treated orally with 10 µg Se/kg body wt. Dams were treated orally with sodium selenite from day 7 to 19 of gestation. Sodium selenite treatment revealed decrease in maternal body weight, reduction in fetal weight, length and number of viable fetuses, increased number of resorbed fetuses and post-implantation loss at the two doses tested. Fetal skeleton showed signs of developmental delay in skull and limbs of the treated groups. Sodium selenite treatment revealed significant reduction of placental and liver weights in treated dams. Sodium selenite-induced oxidative stress in liver tissue of rats as evidenced by increase in lipid peroxidation and glutathione peroxidase activity, while catalase was significantly decreased. Also, increase in DNA fragmentation, marked reduction of hepatic DNA content, and many histopathological changes in the liver were recorded. The results demonstrated that treatment of pregnant rats with sodium selenite at the toxic dosages chosen showed maternal and fetal toxicity that may be concerned with hepatic oxidative stress accompanied with DNA fragmentation and depletion of total DNA content. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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