Evaluation of food intake and Fos expression in serotonergic neurons of raphe nuclei after intracerebroventricular injection of adrenaline in free-feeding rats
| Autor: | Marta Aparecida Paschoalini, Jose Donato, Thais T. Zampieri, Rafael Appel Flores, Ana Paula Dambros Taschetto, Eduardo Simão da Silva, Anderson Savaris Ribas |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Dorsal Raphe Nucleus
Male 0301 basic medicine Serotonin medicine.medical_specialty Median raphe nucleus Epinephrine Gene Expression Adrenergic Neurotransmission Serotonergic RATOS Eating 03 medical and health sciences 0302 clinical medicine Dorsal raphe nucleus Internal medicine medicine Animals Premovement neuronal activity Rats Wistar Molecular Biology Neurons Chemistry General Neuroscience digestive oral and skin physiology Genes fos Rats Infusions Intraventricular 030104 developmental biology Endocrinology Raphe Nuclei Neurology (clinical) Raphe nuclei 030217 neurology & neurosurgery Serotonergic Neurons Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2017.10.021 |
| Popis: | Previous studies indicate that the modification of adrenergic neurotransmission in median raphe nucleus (MRN) enhances or removes an inhibitory influence on food intake, possibly serotonergic, due to a presence of serotonin-producing neurons in that nucleus. Therefore, the aim of this study is evaluated whether the activity of neurons in the MRN and dorsal raphe nucleus (DRN) are affected by intracerebroventricular injection of adrenaline (AD) in free-feeding rats. Male Wistar rats with guide cannulae chronically implanted in the lateral ventricle were injected with AD followed by evaluation of ingestive behavioral parameters. Behavior was monitored and the amount of food ingested was assessed. The highest dose (20 nmol) of AD was the most effective dose in increasing food intake. Subsequently, AD 20 nmol was injected to study neuronal activity indicated by the presence of Fos protein and its co-localization with serotonergic neurons in the MRN and DRN of naive rats with or without access to food during the recording of behavior. The administration of AD 20 nmol increased Fos expression and double labeling with serotonergic neurons in the DRN in rats with access to food, but not in animals without access. No statistically significant changes in Fos expression were observed in the MRN in any of the experimental conditions tested. These results suggest that DRN serotonergic and non-serotonergic neurons are activated by post-prandial signals. In contrast, the absence of Fos expression in the MRN suggests that this nucleus does not participate in the circuit involved in the control of post-prandial satiety. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect