STAT3 enhances intracellular Fas-mediated apoptotic signals in HHUA human endometrial epithelial cells
| Autor: | Kazunori Yukawa, Tetsuji Tanaka, Takahiro Fukumoto, Tao Bai, Hirotoshi Utsunomiya |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
STAT3 Transcription Factor
Cancer Research Programmed cell death Cell Intracellular Space Apoptosis DNA Fragmentation Transfection Biochemistry Cell Line Endometrium Genetics medicine Humans fas Receptor RNA Small Interfering STAT3 Molecular Biology Cell Proliferation biology Cell Membrane Oncostatin M Epithelial Cells Cell cycle Cell biology medicine.anatomical_structure Oncology Gene Knockdown Techniques biology.protein Cancer research Molecular Medicine Cytokines Female Intracellular Signal Transduction |
| Zdroj: | Molecular medicine reports. 4(2) |
| ISSN: | 1791-3004 |
| Popis: | Several endometrial signal transducer and activator of transcription 3 (STAT3)-activating cytokines are reported to be essential for blastocyst implantation, with inhibition of STAT3 activation in the endometrium also reported to prevent implantation. To investigate STAT3 signals in endometrial epithelial cells, the activation and inactivation effects of STAT3 signals were examined in the human endometrial epithelial cell line HHUA, which is thought to retain many of the intracellular signaling pathways found in normal human endometrial epithelial cells. Five STAT3-activating cytokines, IL-11, IL-10, LIF, oncostatin M and leptin, enhanced the Fas-mediated apoptosis of the HHUA cells without any increase in cell surface Fas antigen expression. STAT3 siRNA transfection suppressed STAT3 expression in HHUA cells and significantly inhibited Fas-mediated cell death. These results indicate that intracellular apoptotic signals in HHUA cells are constitutively activated and regulated by STAT3-mediated signals. This apoptosis-promoting effect of STAT3 in HHUA cells is completely different from many previous reports demonstrating anti-apoptotic effects by STAT3 activation. The STAT3 signals in HHUA cells may be specific to the human endometrial epithelial cell lineage in the regulation of blastocyst implantation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|