Low-Dose UVA Radiation-Induced Adaptive Response in Cultured Human Dermal Fibroblasts
| Autor: | Xuemei Li, Zhong-rong Liu, Hulin Chen, Jie Liang, Huilan Yang |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Gel electrophoresis
Article Subject Renewable Energy Sustainability and the Environment DNA repair DNA damage Chemistry lcsh:TJ807-830 Lethal dose lcsh:Renewable energy sources General Chemistry Cell cycle Molecular biology Atomic and Molecular Physics and Optics law.invention Toxicology law Apoptosis General Materials Science sense organs Irradiation Electron microscope |
| Zdroj: | International Journal of Photoenergy, Vol 2012 (2012) |
| ISSN: | 1687-529X 1110-662X |
| Popis: | Objective.To investigate the mechanism of the adaptive response induced by low-dose ultraviolet A (UVA) radiation.Methods.Cultured dermal fibroblasts were irradiated by a lethal dose of UVA (86.4 J/cm2) with preirradiation of single or repetitive low dose of UVA (7.2 J/cm2). Alterations of cellular morphology were observed by light microscope and electron microscope. Cell cycle and cellular apoptosis were assayed by flow cytometer. The extent of DNA damage was determined by single-cell gel electrophoresis (SCGE).Results.The cultured dermal fibroblasts, with pretreatment of single or repetitive irradiation of 7.2 J/cm2UVA relieved toxic reaction of cellular morphology and arrest of cell cycle, decreased apoptosis ratio, reduced DNA chain breakage, and accelerated DNA repair caused by subsequent 86.4 J/cm2UVA irradiation. Compared with nonpretreatment groups, all those differences were significant (P<0.01orP<0.05).Conclusions.The adaptation reaction might depend on the accumulated dose of low-dose UVA irradiation. Low-dose UVA radiation might induce adaptive response that may protect cultured dermal fibroblasts from the subsequent challenged dose of UVA damage. The duration and protective capability of the adaptive reaction might be related to the accumulated dose of low-dose UVA Irradiation. |
| Databáze: | OpenAIRE |
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