Rhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China
| Autor: | Juanjuan Wang, Peng Shi, Weimin Chen, Wenjun Tong, Wenqing Bai, Yunyun Huo, Gehong Wei, En Tao Wang, Fang Wang |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine China Root nodule 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Phylogenetics RNA Ribosomal 16S Botany Medicago sativa Symbiosis Phylogeny Ecology Evolution Behavior and Systematics Phaseolus Base Composition biology Phylogenetic tree Fatty Acids Peas Nucleic Acid Hybridization food and beverages Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Housekeeping gene 030104 developmental biology Genes Bacterial Rhizobium Soybeans Root Nodules Plant |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 69:2049-2056 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003430 |
| Popis: | Two Gram-stain-negative, rod-shaped bacterial strains (C5T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA–glnII–atpD) revealed that C5T and C16 were members of the genus Rhizobium , yet were distinct from known species. The case for strain C5T representing a novel species was supported by genomic results. Pairwise digital DNA–DNA hybridization and average nucleotide identity values were much lower than the proposed and generally accepted species boundaries. The genome-based phylogenetic tree reconstructed by using the up-to-date bacterial core gene set consisting of 92 genes showed that the strains formed a monophyletic branch, further supporting this result. The symbiotic genes of nodC and nifH were identified in both strains; each could nodulate Phaseolus vulgaris and Glycine max but not Leucaena leucocephala, Pisum sativum or Medicago sativa plants. Major cellular fatty acids of C5T were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 58.8 %), C18 : 1 ω7c 11-methyl (14.2 %) and C18 : 0 (8.1 %). The DNA G+C content of C5T was 61.4 mol%. Based on these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species: Rhizobium chutanense sp. nov. The type strain is C5T (=CCTCC AB 2018143T=LMG 30777T). |
| Databáze: | OpenAIRE |
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