Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation
| Autor: | John C. Bischof, Walter C. Low, Joseph Kangas, Kanav Khosla, Qi Shao, Li Zhan, Michael C. McAlpine, Maple Shiao, Shuangzhuang Guo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
droplet vitrification
Materials science Hot Temperature Convective heat transfer Cryoprotectant Cell Survival General Chemical Engineering Science Cell General Physics and Astronomy Medicine (miscellaneous) 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Regenerative medicine conduction cooling Cryopreservation Cell Line Cell therapy medicine Humans General Materials Science Vitrification Cells Cultured Research Articles Nanotubes General Engineering Fibroblasts 021001 nanoscience & nanotechnology 0104 chemical sciences Cold Temperature medicine.anatomical_structure Gold plasmonic laser heating Stem cell cell therapy 0210 nano-technology Biomedical engineering Research Article |
| Zdroj: | Advanced Science Advanced Science, Vol 8, Iss 11, Pp n/a-n/a (2021) |
| ISSN: | 2198-3844 |
| Popis: | Droplet vitrification has emerged as a promising ice‐free cryopreservation approach to provide a supply chain for off‐the‐shelf cell products in cell therapy and regenerative medicine applications. Translation of this approach requires the use of low concentration (i.e., low toxicity) permeable cryoprotectant agents (CPA) and high post cryopreservation viability (>90%), thereby demanding fast cooling and warming rates. Unfortunately, with traditional approaches using convective heat transfer, the droplet volumes that can be successfully vitrified and rewarmed are impractically small (i.e., 180 picoliter) for 400‐fold improvement in warming rates over traditional convective approach. High viability cryopreservation is then demonstrated in a model cell line (human dermal fibroblasts) and an important regenerative medicine cell line (human umbilical cord blood stem cells). This approach opens a new paradigm for cryopreservation and rewarming of dramatically larger volume droplets at lower CPA concentration for cell therapy and other regenerative medicine applications. A novel droplet vitrification system demonstrates high post thaw cell viability (90–95%) with reduced cryoprotectant concentration (i.e., 20%) and improved vitrification throughput (i.e., ≈1000x) using larger (1–4 μL) droplet volumes. Conductive cooling and plasmonic heating approaches are employed and characterized thoroughly to substantially improve the cooling and warming rates compared to traditional convective heat transfer approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text