Expression of precipitating factors of pruritus found in humans in an imiquimod-induced psoriasis mouse model
| Autor: | Kazuko Fujimoto, Akihiro Hisaka, Naotomo Kambe, Katsunori Yamaura, Hiromi Sato, Koichi Ueno, Nobuo Oishi, Noriko Kobayashi, Hiroki Iwata |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Immunology Neuropeptide Imiquimod Endogeny Article Mast cell 03 medical and health sciences 0302 clinical medicine Psoriasis Area and Severity Index Psoriasis medicine lcsh:Social sciences (General) lcsh:Science (General) Multidisciplinary biology business.industry Pruritus medicine.disease 030104 developmental biology medicine.anatomical_structure Nerve growth factor biology.protein lcsh:H1-99 Neurotrophin business 030217 neurology & neurosurgery Neuroscience lcsh:Q1-390 medicine.drug |
| Zdroj: | Heliyon, Vol 5, Iss 6, Pp e01981-(2019) Heliyon |
| ISSN: | 2405-8440 |
| DOI: | 10.1016/j.heliyon.2019.e01981 |
| Popis: | Aims To use a mouse model of imiquimod-induced psoriasis to investigate the relationship between pruritus and mast cells, nerve growth factor (NGF) and endogenous pruritogenic peptides, which are highly expressed in the skin of psoriasis patients. Main methods We developed a mouse model of imiquimod-induced psoriasis and measured the frequency and duration of the model animals’ self-scratching behavior using the SCLABA®-Real real-time scratch counting system. We then harvested the ears and subjected them to toluidine blue staining and real-time PCR. Key findings Topical application of imiquimod increased the Psoriasis Area and Severity Index score as well as the frequency and duration of self-scratching. Regarding internal factors, increases in mast cells number and mRNA expression of NGF and endogenous pruritogenic peptide precursor were confirmed. Significance Self-scratching behavior is accompanied by increased number of mast cells and expression of NGF and endogenous pruritogenic peptides in our imiquimod-induced psoriasis model. The expression of these factors was consistent with the features in patients with pruritic psoriasis, suggesting that our model reflects at least some of the precipitating factors of pruritus found in humans. |
| Databáze: | OpenAIRE |
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