Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena
| Autor: | Akira Arisawa, Hitosi Agematu, Shinji Hirosue, Yoshikazu Fujii, Naoki Matsumoto, Tadashi Fujii |
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| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Stereochemistry Electrospray ionization medicine.disease_cause Hydroxylation Applied Microbiology and Biotechnology Biochemistry Redox Analytical Chemistry Catalysis Cell-free system chemistry.chemical_compound Industrial Microbiology Cytochrome P-450 Enzyme System medicine Escherichia coli Oleanolic Acid Molecular Biology Oleanolic acid Nuclear Magnetic Resonance Biomolecular biology Cell-Free System Molecular Structure Organic Chemistry Cytochrome P450 General Medicine Recombinant Proteins Actinobacteria chemistry biology.protein Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 70(9) |
| ISSN: | 0916-8451 |
| Popis: | A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%. |
| Databáze: | OpenAIRE |
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