Passage‐dependent expression of STRO‐1 in human gingival mesenchymal stem cells
| Autor: | Rajasekaran Subbarayan, Suresh Ranga Rao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Gingiva CD34 Biology Biochemistry Flow cytometry 03 medical and health sciences 0302 clinical medicine Gene expression medicine Humans CD90 Molecular Biology Transcription factor medicine.diagnostic_test Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Nestin Molecular biology 030104 developmental biology 030220 oncology & carcinogenesis Antigens Surface Thy-1 Antigens Biomarkers Transcription Factors |
| Zdroj: | Journal of Cellular Biochemistry. 120:2810-2815 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.27674 |
| Popis: | The expression of STRO-1, the essential mesenchymal stem cell marker, was found to decrease with advancing passages in few tissues. Because STRO-1 was identified and isolated from human gingiva, we were interested to know its status after a few passages. Human gingival mesenchymal stem cells (HGMSCs) were isolated from human gingiva. Flow cytometry was carried out with STRO-1, mesenchymal stem cell (MSC) positive marker CD73, and negative marker CD34/CD45. Samples were also subjected to CD90 and STRO-1 immunofluorescence staining. Gene expression was carried out for transcription factors OCT-4, NANOG, and NESTIN. The results showed a gradual decrease in STRO-1 and transcription factor expression with an increase in passage numbers. MSC positive marker CD73 was consistently expressed in all the passages. Negative markers were absent in all the passages. We conclude that STRO-1 may be a useful marker to isolate undifferentiated (potent) mesenchymal cells from gingiva. |
| Databáze: | OpenAIRE |
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