Differential expression of inflammasome regulatory transcripts in periodontal disease
| Autor: | Yvonne L. Kapila, Kübra Aral, Paul R. Cooper, Cüneyt Asım Aral, Michael R. Milward, Afig Berdeli, Beyza Karadede Ünal, Eynar Berdeli |
|---|---|
| Přispěvatelé: | Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Ubiquitin-Protein Ligases Interleukin-1beta periodontal disease tripartite motif proteins Pyrin domain Proinflammatory cytokine Pathogenesis 03 medical and health sciences AIM2 Gingivitis 0302 clinical medicine NLR Family Pyrin Domain-Containing 3 Protein Medicine Aggressive periodontitis Humans pyrin domain inflammasomes caspase activation and recruitment domains business.industry Caspase 1 Inflammasome 030206 dentistry medicine.disease Chronic periodontitis Molecular biology DNA-Binding Proteins 030104 developmental biology Aggressive Periodontitis Chronic Periodontitis Periodontics Female medicine.symptom business medicine.drug Transcription Factors |
| Popis: | Background the inflammasome modulates the release of key proinflammatory cytokines associated with periodontal disease pathogenesis. the aim of this study was to evaluate the expression of proteins that regulate the inflammasome, namely pyrin domain-only proteins (POPs), caspase activation recruitment domain (CARD)-only proteins, and tripartite motif-containing (TRIM) proteins, in periodontal diseases. Methods A total of 68 participants (34 males and 34 females) were divided into four groups, including periodontal health (H), gingivitis (G), chronic periodontitis (CP), and aggressive periodontitis (AgP) based on clinical parameters. Gingival tissue samples were obtained from all participants for reverse transcription polymerase chain reaction (RT-PCR)-based gene expression analyses of molecules that regulate the inflammasome, including apoptosis-associated speck-like protein (ASC) containing CARD, caspase-1, interleukin-1 beta (IL-1 beta), interleukin-18 (IL-18), nucleotide-binding domain, leucine rich family (NLR) pyrin domain containing 3 (NLRP3), NLR family pyrin domain containing 2 (NLRP2), AIM2 (absent in melanoma 2), POP1, POP2, CARD16, CARD18, TRIM16, and TRIM20 by RT-PCR. Results NLRP3 and IL-1 beta were upregulated in the G, CP, and AgP groups compared with group H (P < 0.05). AIM2 was downregulated in the CP group compared with the H, G, and AgP groups (P < 0.05). TRIM20, TRIM16, and CARD18 were downregulated in the G, CP, and AgP groups compared with the H group (P < 0.05). POP1 and POP2 were downregulated in the CP and AgP, and AgP and G groups, respectively (P < 0.05). Conclusion Active periodontal disease may result in downregulation of inflammasome regulators that may increase the activity of NLRP3 and IL-1 beta in periodontal disease. Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) This study was not supported by any financial organization. the corresponding author of this study (Kubra Aral) was supported with a scholarship by the Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkey. the authors report no conflicts of interest related to this study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|