Effect of vitamin A supplementation on gut microbiota in children with autism spectrum disorders - a pilot study
| Autor: | Xue-Qin Xiong, Xi Lai, Juan Liu, Min Guo, Xiao Liu, Nali Hou, Qian Cheng, Xiao-Hua Liang, Ting Cui, Tingyu Li, Jie Chen, Ting Yang, Shu Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Male medicine.medical_specialty Non-Randomized Controlled Trials as Topic Autism Spectrum Disorder lcsh:QR1-502 Child Behavior Pilot Projects Gut microbiota Gut flora Microbiology lcsh:Microbiology 03 medical and health sciences Feces 0302 clinical medicine Internal medicine RNA Ribosomal 16S mental disorders medicine Humans Single-Blind Method RNA Messenger Vitamin A Child biology Infant Autism spectrum disorders medicine.disease biology.organism_classification Institutional review board ADP-ribosyl Cyclase 1 Gastrointestinal Microbiome Clinical trial 030104 developmental biology Autism spectrum disorder Child Preschool Immunology Dietary Supplements Childhood Autism Rating Scale Autism Female Dysbiosis 030217 neurology & neurosurgery Biomarkers Research Article Follow-Up Studies |
| Zdroj: | BMC Microbiology BMC Microbiology, Vol 17, Iss 1, Pp 1-14 (2017) |
| ISSN: | 1471-2180 |
| Popis: | Background Dysbiosis of gut microbiota are commonly reported in autism spectrum disorder (ASD) and may contribute to behavioral impairment. Vitamin A (VA) plays a role in regulation of gut microbiota. This study was performed to investigate the role of VA in the changes of gut microbiota and changes of autism functions in children with ASD. Results Sixty four, aged 1 to 8 years old children with ASD completed a 6-month follow-up study with VA intervention. High-performance liquid chromatography was used to assess plasma retinol levels. The Autism Behaviour Checklist (ABC), Childhood Autism Rating Scale (CARS) and Social Responsiveness Scale (SRS) were used to assess autism symptoms. CD38 and acid-related orphan receptor alpha (RORA) mRNA levels were used to assess autism-related biochemical indicators’ changes. Evaluations of plasma retinol, ABC, CARS, SRS, CD38 and RORA mRNA levels were performed before and after 6 months of intervention in the 64 children. Illumina MiSeq for 16S rRNA genes was used to compare the differences in gut microbiota before and after 6 months of treatment in the subset 20 of the 64 children. After 6 months of intervention, plasma retinol, CD38 and RORA mRNA levels significantly increased (all P 0.05) in the 64 children. Meanwhile, the proportion of Bacteroidetes/Bacteroidales significantly increased and the proportion of Bifidobacterium significantly decreased in the subgroup of 20 (all false discovery rate (FDR) q |
| Databáze: | OpenAIRE |
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