A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions
| Autor: | James R. Fouts, Roberta J. Pohl |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Male
Biophysics Biochemistry chemistry.chemical_compound Drug Stability beta-Naphthoflavone Microsomes Oxazines Cytochrome P-450 CYP1A1 Animals Centrifugation Bovine serum albumin Molecular Biology Incubation Edetic Acid Benzoflavones Oxidase test Chromatography biology Serum Albumin Bovine Cell Biology Metabolism Fluorescence Rats Spectrometry Fluorescence chemistry biology.protein Microsome Methanol Oxidoreductases |
| Zdroj: | Analytical Biochemistry. 107:150-155 |
| ISSN: | 0003-2697 |
| Popis: | The formation of resorufin from 7-ethoxyresorufin by microsomal mixed-function oxidase may be assayed rapidly by precipitating the protein in incubation mixtures with methanol and measuring the fluorescence of resorufin in the supernatant after centrifugation. Conditions are described for minimizing the effects of the instability of resorufin and of inhibition by the product. Higher activity and linear plots of microsomal protein concentration versus activity are obtained by including bovine serum albumin or ethylenediamine-tetraacetate in the incubation mixture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|