Cholinergic Deafferentation of Dorsal Hippocampus by Fimbria-Fornix Lesioning Differentially Regulates Subtypes (m1-m5) of Muscarinic Receptors
| Autor: | Stephen J. Wall, Lawrence F. Kromer, Barry B. Wolfe |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Suction Biology Hippocampus Biochemistry Choline Choline O-Acetyltransferase Cellular and Molecular Neuroscience Postsynaptic potential Internal medicine Muscarinic acetylcholine receptor medicine Animals Hippocampus (mythology) Receptor Immunosorbent Techniques Binding Sites Muscarinic acetylcholine receptor M2 Muscarinic acetylcholine receptor M1 Denervation Receptors Muscarinic Choline acetyltransferase Rats Quinuclidinyl Benzilate Endocrinology Cholinergic Female |
| Zdroj: | Journal of Neurochemistry. 62:1345-1351 |
| ISSN: | 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1994.62041345.x |
| Popis: | Unilateral aspiration lesions of the rostral supracallosal stria/cingulum bundle and fimbria-fornix were performed on adult female rats. Ten and 24 days post lesioning, an elevation (17%; p < 0.01) of total muscarinic receptors was observed in lesioned versus control hippocampi. By using antisera selective for each of the five molecularly defined subtypes (m1-m5) of muscarinic receptors, significant changes were observed in the levels of expression for at least four receptor proteins. Three receptor subtypes increased in density: m1 by 14% (from 943 to 1,078 fmol/mg); m3 by 77% (from 150 to 268 fmol/mg); and m4 by 29% (from 220 to 285 fmol/mg). In contrast, a 22% decrease in the density of m2 receptors was found (from 220 to 173 fmol/mg). Detectable levels of m5 receptors were low in the hippocampus (approximately 1% of total receptors), and reliable measurements were not obtained. The directions of these changes are likely to be related to the pre- or postsynaptic localization of these receptor subtypes. |
| Databáze: | OpenAIRE |
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