Failure of Schwann cells as supporting cells for adult neural progenitor cell grafts in the acutely injured spinal cord
| Autor: | Massimiliano Caioni, Norbert Weidner, Maurice Vroemen, Ulrich Bogdahn |
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| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Cell Survival Schwann cell Biology Pathology and Forensic Medicine Myelin Cell Movement otorhinolaryngologic diseases medicine Animals Progenitor cell Remyelination Axon Fluorescent Antibody Technique Indirect Spinal cord injury Myelin Sheath Spinal Cord Injuries Neurons Cysts Stem Cells Cell Biology medicine.disease Neural stem cell Axons Coculture Techniques Rats Inbred F344 Nerve Regeneration Rats stomatognathic diseases Disease Models Animal surgical procedures operative medicine.anatomical_structure nervous system Female Schwann Cells Stem cell Neuroscience Stem Cell Transplantation |
| Zdroj: | Cell and tissue research. 327(1) |
| ISSN: | 0302-766X |
| Popis: | Adult neural progenitor cells (NPC) co-grafted with fibroblasts replace cystic lesion defects and promote cell-contact-mediated axonal regeneration in the acutely injured spinal cord. Fibroblasts are required as a platform to maintain NPC within the lesion; however, they are suspected to create an inhospitable milieu for regenerating central nervous system (CNS) axons. Therefore, we thought to replace fibroblasts by primary Schwann cells, which might serve as a superior scaffold to maintain NPC within the lesion and might further enhance axon regrowth and remyelination following spinal cord injury. Adult rats underwent a cervical dorsal column transection immediately followed by transplantation of either NPC/Schwann cell or NPC/Schwann cell/fibroblast co-grafts. Animals receiving Schwann cell or fibroblast grafts alone, or Schwann cell/fibroblast co-grafts served as controls. At 3 weeks after injury/transplantation, histological analysis revealed that only fibroblast-containing grafts were able to replace the cystic lesion defect. In both co-cultures and co-grafts, Schwann cells and NPC were segregated. Almost all NPC migrated out of the graft into the adjacent host spinal cord. As a consequence, only peripheral-type myelin, but no CNS-type myelin, was detected within co-grafts containing NPC/Schwann cells. Corticospinal axon regeneration into Schwann-cell-containing co-grafts was reduced. Taken together, Schwann cells within NPC grafts contribute to remyelination. However, Schwann cells fail as a supporting platform to maintain NPC within the graft and impair CNS axon regeneration; this makes them an unfavorable candidate to support/augment NPC grafts following spinal cord injury. |
| Databáze: | OpenAIRE |
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