Promising PEGylated cationic dendrimers for delivery of miRNAs as a possible therapy against HIV-1 infection
| Autor: | Ignacio Rodriguez-Izquierdo, E. Royo-Rubio, M. A. Muñoz-Fernández, Tania Lozano-Cruz, M. Moreno-Domene, F. J. de la Mata, Rafael Gómez, Jose L. Jimenez |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Dendrimers
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) HIV Infections Bioengineering HIV-1 infection Applied Microbiology and Biotechnology Cell Line Polyethylene Glycols Flow cytometry law.invention Drug Delivery Systems Confocal microscopy law Cations Nucleic Acids Dendrimer microRNA Medical technology medicine Humans Particle Size R855-855.5 Cytotoxicity Inhibition medicine.diagnostic_test Chemistry Research RNA Molecular medicine microRNAs Cell biology HIV-1 Leukocytes Mononuclear Nucleic acid Molecular Medicine Carbosilane dendrimers Delivery TP248.13-248.65 Biotechnology |
| Zdroj: | Journal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-16 (2021) Journal of Nanobiotechnology |
| ISSN: | 1477-3155 |
| Popis: | Background The appearance of resistance against new treatments and the fact that HIV-1 can infect various cell types and develop reservoirs and sanctuaries makes it necessary to develop new therapeutic approaches to overcome those failures. Results Studies of cytotoxicity, genotoxicity, complexes formation, stability, resistance, release and particle size distribution confirmed that G2-SN15-PEG, G3-SN31-PEG, G2-SN15-PEG-FITC and G3-SN31-PEG-FITC dendrimers can form complexes with miRNAs being biocompatible, stable and conferring protection to these nucleic acids. Confocal microscopy and flow cytometry showed effective delivery of these four dendrimers into the target cells, confirming their applicability as delivery systems. Dendriplexes formed with the dendrimers and miRNAs significantly inhibited HIV-1 infection in PBMCs. Conclusions These dendrimers are efficient delivery systems for miRNAs and they specifically and significantly improved the anti-R5-HIV-1 activity of these RNA molecules. Graphic Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink