Epigenetic activation of the prostaglandin receptor EP4 promotes resistance to endocrine therapy for breast cancer
Autor: | Keith F. Decker, Margaret Akinhanmi, Kyle L. Jung, Jeremy Hoog, César Sánchez, Nathan D. VanderKraats, James I. McDonald, John R. Edwards, Jeffrey F. Hiken, Lisa E. Rois, Matthew J. Ellis |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Protein-Arginine N-Methyltransferases Cancer Research medicine.medical_specialty Estrogen receptor Antineoplastic Agents Breast Neoplasms Biology Molecular oncology Article Epigenesis Genetic Epigenome 03 medical and health sciences Breast cancer Growth factor receptor Internal medicine Breast Cancer Genetics medicine Humans Epigenetics Prostaglandin Receptor skin and connective tissue diseases Prostaglandin receptor Molecular Biology Cell Proliferation EP4 Aromatase Inhibitors Estrogen Receptor alpha Endocrine Therapy Cancer DNA Neoplasm DNA Methylation Cell cycle medicine.disease 3. Good health Gene Expression Regulation Neoplastic 030104 developmental biology Endocrinology Drug Resistance Neoplasm MCF-7 Cells Cancer research Female lipids (amino acids peptides and proteins) Receptors Prostaglandin E EP4 Subtype Signal Transduction |
Zdroj: | Oncogene |
ISSN: | 1476-5594 0950-9232 |
Popis: | Approximately 75% of breast cancers express estrogen receptor α (ERα) and depend on estrogen signals for continued growth. Aromatase inhibitors (AIs) prevent estrogen production and inhibit estrogen receptor signaling, resulting in decreased cancer recurrence and mortality. Advanced tumors treated with AIs almost always develop resistance to these drugs via the up-regulation of alternative growth signals. The mechanisms that drive this resistance—especially epigenetic events that alter gene expression—are however not well understood. Genome-wide DNA methylation and expression analysis of cell line models of acquired aromatase inhibitor resistance indicated that prostaglandin E2 receptor 4 (PTGER4) is up-regulated after demethylation in resistant cells. Knockdown and inhibitor studies demonstrate that PTGER4 is essential for estrogen independent growth. Our exploratory analysis of downstream signaling indicates that PTGER4 likely promotes AI resistance via ligand independent activation of the ERα-cofactor CARM1. We believe that we have discovered a novel epigenetic mechanism for altering cell signaling and acquiring endocrine therapy resistance. Our findings indicate that PTGER4 is a potential drug target in AI resistant cancers. Additionally, the epigenetic component of PTGER4 regulation suggests that further study of PTGER4 may yield valuable insights into how DNA methylation-targeted diagnoses and treatments can improve AI resistant breast cancer treatment. |
Databáze: | OpenAIRE |
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