Phenotypic and functional properties of dedifferentiated fat cells derived from infrapatellar fat pad
| Autor: | Koji Tanimoto, Taro Matsumoto, Yuki Nagaoka, Tomohiko Kazama, Chii Yamamoto, Koichiro Kano, Masahiro Nagaoka, Shu Saito, Yasuaki Tokuhashi, Kazuyoshi Nakanishi |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Adipose-derived stem cells
Medicine (General) MSCs mesenchymal stem cells RT-PCR reverse transcription-polymerase chain reaction DEGs differentially expressed genes PBS phosphate-buffered saline Biomedical Engineering Dedifferentiated fat cells Biomaterials R5-920 FBS fetal bovine serum IFP infrapatellar fat pad FACS fluorescence-activated cell sorting Adipocytes PCA principal component analysis QH573-671 HLA human leukocyte antigen WST-1 water soluble tetrazolium-1 SC subcutaneous adipose tissue TBS Tris-buffered saline DFATs dedifferentiated fat cells DFs dermal fibroblasts OA osteoarthritis Infrapatellar fat pad Original Article BSA bovine serum albumin Cytology ASCs adipose tissue-derived stem cells Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 19, Iss, Pp 35-46 (2022) Regenerative Therapy |
| ISSN: | 2352-3204 |
| Popis: | Introduction: Mature adipocyte-derived dedifferentiated fat cells (DFATs) are mesenchymal stem cell (MSC)-like cells with high proliferative ability and multilineage differentiation potential. In this study, we first examined whether DFATs can be prepared from infrapatellar fat pad (IFP) and then compared phenotypic and functional properties of IFP-derived DFATs (IFP-DFATs) with those of subcutaneous adipose tissue (SC)-derived DFATs (SC-DFATs). Methods: Mature adipocytes isolated from IFP and SC in osteoarthritis patients (n = 7) were cultured by ceiling culture method to generate DFATs. Obtained IFP-DFATs and SC-DFATs were subjected to flow cytometric and microarray analysis to compare their immunophenotypes and gene expression profiles. Cell proliferation assay and adipogenic, osteogenic, and chondrogenic differentiation assays were performed to evaluate their functional properties. Results: DFATs could be prepared from IFP and SC with similar efficiency. IFP-DFATs and SC-DFATs exhibited similar immunophenotypes (CD73+, CD90+, CD105+, CD31-, CD45-, HLA-DR-) and tri-lineage (adipogenic, osteogenic, and chondrogenic) differentiation potential, consistent with the minimal criteria for defining MSCs. Microarray analysis revealed that the gene expression profiles in IFP-DFATs were very similar to those in SC-DFATs, although there were certain number of genes that showed different levels of expression. The proliferative activity in IFP-DFATs was significantly (p |
| Databáze: | OpenAIRE |
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