Molecular characterization of carrier rabies isolates
| Autor: | Makonnen Fekadu, Theodore G. Schurr, Cynthia K. Warner |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Sequence analysis Rabies Molecular Sequence Data Biology medicine.disease_cause Virus law.invention Restriction map Dogs law Virology medicine Animals Polymerase chain reaction Phylogeny Base Sequence Rabies virus medicine.disease Nucleoprotein Infectious Diseases Carrier State DNA Viral Restriction fragment length polymorphism Peptides Polymorphism Restriction Fragment Length |
| Zdroj: | Virus research. 41(2) |
| ISSN: | 0168-1702 |
| Popis: | We compared the genomes of nine dog rabies virus isolates using two molecular methods. The viruses used in the comparison included three Ethiopian rabies strains from carrier dogs, a street strain from a rabid dog from the same geographic area, two saliva isolates made from an experimentally infected carrier dog, the virus isolated from the tonsil of this carrier dog at necropsy, and two laboratory strains. We produced overlapping polymerase chain reaction (PCR) segments spanning 97% of the genome. Restriction analysis of these PCR products with AvaII, Bcll, and BamHI detected 39 variable sites representing 668 nucleotides (nt) or 5.5% of the genome. We also compared the DNA and the deduced peptide sequences of a 200-nt segment of the 3' end of the rabies nucleoprotein gene. Previous work with these Ethiopian carrier viruses and the endemic street strain had failed to show any differences among them. Both restriction mapping and sequence analysis of 200 nt of the nucleoprotein gene allowed us to individually identify these isolates. Phylogenetic analyses of these data sets showed only the two saliva isolates of the experimentally infected carrier dog to be identical. Each of the viruses in this study, including the one isolated from the tonsil of the experimentally infected carrier dog, could be distinguished by these techniques. |
| Databáze: | OpenAIRE |
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