Antibiotic resistance profile of Enterococcus faecium and Enterococcus faecalis isolated from broiler cloacal samples
| Autor: | Murat Yildirim, Nilgün Ünal, Şinasi Aşkar |
|---|---|
| Přispěvatelé: | Kırıkkale Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antibiotic resistance broiler Enterococcus faecalis Enterococcus faecium van A Antibiotic resistance medicine.drug_class Tetracycline Science Enterococcus faecium 030106 microbiology Erythromycin Drug resistance broiler vanA Enterococcus faecalis Macrolide Antibiotics Microbiology 03 medical and health sciences medicine Fen General Veterinary biology biochemical phenomena metabolism and nutrition biology.organism_classification Vancomycin medicine.drug |
| Zdroj: | Volume: 41, Issue: 2 199-203 Turkish Journal of Veterinary and Animal Sciences |
| ISSN: | 1300-0128 1303-6181 |
| Popis: | WOS: 000399874200009 The present study was performed to isolate and identify Enterococcus spp. from broiler cloacal samples to species level, to determine their resistance patterns to various antibiotics, and to detect vancomycin resistance genes. Cloacal samples of broilers collected from slaughterhouses were inoculated in Slanetz and Bartley agars with and without vancomycin (6 mu g/mL). Antibiotic resistance/susceptibility testing of the isolated and identified enterococci was performed by using the disk diffusion test. Multiplex PCR was used to identify the species and to detect vancomycin resistance genes. The majority of the isolated enterococci was Enterococcus faecium (60.43%, n = 142) and Enterococcus faecalis (33.62%, n = 79). E. casseliflavus and E. gallinarum were identified from 8 (3.42%) and 6 (2.56%) isolates, respectively. It was found that 88.9% of the enterococci were resistant to tetracycline and 83.4% of them were resistant to erythromycin. As a result, none of the strains isolated from cloacal samples of broilers carried the vanA and vanB genes. It was observed that 54.9% of E. faecium isolates and 78.4% of E. faecalis isolates were multidrug resistant (resistant to 3 or more antibiotic groups). The lack of vancomycin-resistant Enterococcus among the enterococci isolates was important for public health. Kirikkale UniversityKirikkale University [2012 / 46] The authors thank Kirikkale University (project number: 2012 / 46) for supporting this work. |
| Databáze: | OpenAIRE |
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