Comparison of the X-ray Structure of Native Rubredoxin from Pyrococcus furiosus with the NMR Structure of the Zinc-Substituted Protein
| Autor: | Douglas C. Rees, Jae-Bum Park, Michael W. W. Adams, Dennis R. Hare, Paul R. Blake, Michael W. Day, Barbara T. Hsu, Leemor Joshua-Tor, Michael F. Summers |
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| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Models
Molecular Magnetic Resonance Spectroscopy biology Rubredoxins chemistry.chemical_element Zinc Nuclear magnetic resonance spectroscopy biology.organism_classification Archaea Biochemistry Protein Structure Secondary Hyperthermophile Crystallography Protein structure X-Ray Diffraction chemistry Rubredoxin X-ray crystallography Pyrococcus furiosus Amino Acid Sequence Molecular Biology Peptide sequence Research Article |
| Popis: | The three-dimensional X-ray structures of the oxidized and reduced forms of rubredoxin from Pyrococcus furiosus, determined at -161 degrees C, and the NMR structure of the zinc-substituted protein, determined in solution at 45 degrees C, are compared. The NMR and X-ray structures, which were determined independently, are very similar and lead to similar conclusions regarding the interactions that confer hyperthermostability. |
| Databáze: | OpenAIRE |
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