Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes
| Autor: | Anne van der Meij, Joeri A. J. Wondergem, Dennis Claessen, Joost Willemse, Eveline Ultee, Zheren Zhang, Gilles P. van Wezel, Karina Ramijan, Ariane Briegel, Doris Heinrich |
|---|---|
| Přispěvatelé: | Publica |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sucrose Osmotic shock Science 030106 microbiology Adaptation Biological L Forms General Physics and Astronomy Penicillins Spheroplasts Bacterial Physiological Phenomena Article General Biochemistry Genetics and Molecular Biology Zellen Cell wall 03 medical and health sciences Bacterial Proteins Cell Wall Osmotic Pressure RNA Ribosomal 16S Osmotic pressure lcsh:Science Phylogeny Mycelium Microbial Viability Multidisciplinary Whole Genome Sequencing biology Chemistry fungi Stress induced food and beverages RNA General Chemistry biology.organism_classification Cell biology Actinobacteria 030104 developmental biology lcsh:Q Sequence Alignment Gene Deletion Bacteria |
| Zdroj: | Nature Communications Nature Communications, 9, 5164 Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-07560-9 |
| Popis: | The cell wall is a shape-defining structure that envelopes almost all bacteria and protects them from environmental stresses. Bacteria can be forced to grow without a cell wall under certain conditions that interfere with cell wall synthesis, but the relevance of these wall-less cells (known as L-forms) is unclear. Here, we show that several species of filamentous actinomycetes have a natural ability to generate wall-deficient cells in response to hyperosmotic stress, which we call S-cells. This wall-deficient state is transient, as S-cells are able to switch to the normal mycelial mode of growth. However, prolonged exposure of S-cells to hyperosmotic stress yields variants that are able to proliferate indefinitely without their cell wall, similarly to L-forms. We propose that formation of wall-deficient cells in actinomycetes may serve as an adaptation to osmotic stress. Bacteria can be forced to grow without cell wall if cell wall synthesis is inhibited. Here Ramijan et al. show that, in filamentous actinomycetes, hyperosmotic stress induces formation of wall-deficient cells that can switch to normal mycelial growth, or mutate and proliferate indefinitely as wall-less forms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|