Au-NPs signal amplification ultra-sensitivity optical microfiber interferometric biosensor
| Autor: | Wenfu Lin, Ruitao Xiao, Li-Peng Sun, Bai-Ou Guan, Jie Li, Jun Ma, Wei Wang, Zhenru Li, Hanglin Lu, Yan Huang, Peiyuan Liu, Runjia Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
business.product_category Metal Nanoparticles Biosensing Techniques 02 engineering and technology 01 natural sciences 010309 optics Optics Limit of Detection 0103 physical sciences Microfiber Surface plasmon resonance Detection limit business.industry Oligonucleotide Optical Devices DNA Equipment Design 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Interferometry Fiber optic sensor Colloidal gold Optoelectronics Gold 0210 nano-technology business Biosensor |
| Zdroj: | Optics Express. 29:13937 |
| ISSN: | 1094-4087 |
| DOI: | 10.1364/oe.424878 |
| Popis: | An optical microfiber interferometric biosensor for the low concentration detection of sequence-specific deoxyribonucleic acid (DNA) based on signal amplification technology via oligonucleotides linked to gold nanoparticles (Au-NPs) is proposed and experimentally analyzed. The sensor uses a “sandwich” detection strategy, in which capture probe DNA (DNA-c) is immobilized on the surface of the optical microfiber interferometer, the reporter probe DNA (DNA-r) is immobilized on the surface of Au-NPs, and the DNA-c and DNA-r are hybridized to the target probe DNA (DNA-t) in a sandwich arrangement. The dynamic detection of the DNA-t was found to range from 1.0×10−15 M to 1.0×10−8 M, and the limit of detection (LOD) concentration was 1.32 fM. This sensor exhibited not only a low LOD but also excellent selectivity against mismatched DNA-t, and it can be further developed for application in various sensing platforms. |
| Databáze: | OpenAIRE |
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