Spray-Capillary-Based Capillary Electrophoresis Mass Spectrometry for Metabolite Analysis in Single Cells
| Autor: | Mulin Fang, Zhe Wang, Lushuang Huang, Si Wu, Kenneth J. Smith, Kellye A. Cupp-Sutton |
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| Rok vydání: | 2021 |
| Předmět: |
Accuracy and precision
Spectrometry Mass Electrospray Ionization Chromatography Capillary action Chemistry Sample (material) Electrospray ionization 010401 analytical chemistry Electrophoresis Capillary 010402 general chemistry Mass spectrometry 01 natural sciences Capillary electrophoresis–mass spectrometry Mass Spectrometry Article 0104 chemical sciences Analytical Chemistry Electrophoresis Sample collection |
| Zdroj: | Anal Chem |
| ISSN: | 1520-6882 |
| Popis: | Single-cell capillary electrophoresis mass spectrometry (CE-MS) is a promising platform to analyze cellular contents and probe cell heterogeneity. However, current single-cell CE-MS methods often rely on offline microsampling processes and may demonstrate low sampling precision and accuracy. We have recently developed an electrospray-assisted device, spray-capillary, for low-volume sample extraction. With the spray-capillary, low-volume samples (pL-nL) are drawn into the sampling end of the device, which can be used directly for CE separation and online MS detection. Here, we redesigned the spray-capillary by utilizing a capillary with a |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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