| Autor: |
Amir Niaraki, Marilyn C. McNamara, Reza Montazami, Nicole N. Hashemi |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
ACS applied bio materials. 5(1) |
| ISSN: |
2576-6422 |
| Popis: |
Understanding the changes in the electrochemical properties of neural cells upon exposure to stress factors imparts vital information about the conditions prior to their death. This study presents a graphene-based biosensor for real-time monitoring of N27 rat dopaminergic neural cells which characterizes cell adhesion and cytotoxicity factors through impedance spectroscopy. The aim was to monitor the growth of the entire cell network via a nonmetallic flexible electrode array. Therefore, a water-based graphene solution was formulized as a conductive ink, 3D-printed into a flexible substrate through an electrohydrodynamic approach, resulting in electrodes with a conductivity of 6750 s/m. The presented high-throughput method enabled microscale monitoring of the entire cell network via the design of PDMS-based growth channels. The electrical resistance of the cell network was measured continuously along with their network density, constituting a mean density of 1890 cell/mm |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
