Nonradioactive Cell Assay for the Evaluation of Modular Prostate-Specific Membrane Antigen Targeting Ligands via Inductively Coupled Plasma Mass Spectrometry
| Autor: | Malte Holzapfel, Natalija Peric, Markus Fischer, Indranath Chakraborty, Marina Mutas, Sharah Chandralingam, Torben Segelke, John V. Frangioni, Carla von Salisch, Wolfgang J. Parak, Wolfgang Maison |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Glutamate Carboxypeptidase II
Male Cell urologic and male genital diseases Sensitivity and Specificity 01 natural sciences 03 medical and health sciences Europium Cell Line Tumor Drug Discovery LNCaP medicine Glutamate carboxypeptidase II Humans Inductively coupled plasma mass spectrometry 030304 developmental biology Membrane antigen Targeting ligands 0303 health sciences Chemistry Ligand Spectrophotometry Atomic Radiochemistry Prostatic Neoplasms In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry medicine.anatomical_structure Antigens Surface Molecular Medicine Biological Assay |
| Zdroj: | Journal of Medicinal Chemistry. 62:10912-10918 |
| ISSN: | 1520-4804 0022-2623 |
| Popis: | The development of novel prostate-specific membrane antigen (PSMA)-targeted radioactive theranostic agents is currently limited to facilities capable of working with high-energy radioisotopes. Even preselection of lead structures in vitro relies mostly on radioactive assays with PSMA(+) LNCaP and PSMA(-) PC-3 cells. Assays utilizing radioisotopes are time consuming, costly, and limit discovery to a small group of scientists with special facilities. Nonradioactive alternatives are therefore needed in the field. In this paper, we describe an inductively coupled plasma mass spectrometry (ICP-MS)-based method for the evaluation of PSMA-targeting ligands conjugated to DOTA-chelates of Europium. This method is based on LNCaP and PC-3 cells and has been validated with the well-established targeting ligand PSMA-617. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|