Purification of Lectin from Larvae of the fly,Musca domestica, and in Vitro Anti-Tumor Activity in MCF-7 Cells
| Autor: | Q. Zhao, Bin Zeng, Chengjian Xu, Chunling Wang, Xiaohong Cao, Meifang Lu, De-zhi Mao, Z. Huo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Blotting
Western Apoptosis DNA Fragmentation Biology Chromatography Affinity Article Anti-tumor activity chemistry.chemical_compound Western blot Cell Line Tumor Houseflies Lectins hemic and lymphatic diseases In Situ Nick-End Labeling medicine Animals Humans neoplasms Chromatography High Pressure Liquid Musca domestica larvae Cell Proliferation Dose-Response Relationship Drug medicine.diagnostic_test Caspase 3 Cell growth Acridine orange General Medicine Flow Cytometry Molecular biology In vitro Blot chemistry Cell culture Larva Insect Science DNA fragmentation Calcium Female Lectin |
| Zdroj: | Journal of Insect Science |
| ISSN: | 1536-2442 |
| DOI: | 10.1673/031.010.14124 |
| Popis: | A new lectin was purified from larvae of the fly, Musca domestica L. (Diptera: Muscidae) (MLL-2, 38 kDa) using affinity chromatography and HPLC. Anti-tumor activity of MLL-2 was demonstrated by its inhibition of proliferation of human breast cancer (MCF-7) cells in a time-and dose-dependent manner. The results of acridine orange staining indicated that MLL-2 caused apoptosis in MCF-7 cells. DNA fragmentation in MCF-7 cells has been detected by TUNEL. Flow cytometric analysis also demonstrated that MLL-2 caused dose-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. The MLL-2 induced a sustained increase in concentration of intracellular free calcium. Western blot revealed that MLL-2 induced apoptosis in MCF-7 cells was associated with typical apoptosis proteins in the mitochondrial pathway. In addition, the caspase-3 activity in MCF-7 cells treated with MLL-2 for 48 hours was significantly increased compared to controls (407.4 ± 3.0 vs. 1749.2 ± 6.0, P |
| Databáze: | OpenAIRE |
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