Detection of circulating tumor cells in cervical cancer using a conditionally replicative adenovirus targeting telomerase‐positive cells
| Autor: | Hideki Hayakawa, Takeshi Obata, Yasunari Mizumoto, Kyosuke Kagami, Rena Yamazaki, Takashi Iizuka, Mitsuhiro Nakamura, Yukiko Bono, Toshiyoshi Fujiwara, Subaru Myojyo, Fuminori Sakurai, Masahiro Takakura, Jyunpei Iwadare, Toshiyuki Sasagawa, Takeo Matsumoto, Hiroyuki Mizuguchi, Yasuo Urata, Satoru Kyo, Kentaro Nakayama, Shunsuke Orisaka, Hiroshi Fujiwara |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Telomerase cervical cancer Adenoviridae Infections education Uterine Cervical Neoplasms telomerase Virus Replication circulating tumor cell Virus Adenoviridae 03 medical and health sciences Cytokeratin 0302 clinical medicine Circulating tumor cell human papilloma virus hemic and lymphatic diseases Cell Line Tumor Medicine Humans neoplasms Genetics Genomics and Proteomics Cervical cancer biology business.industry cytokeratin Cancer General Medicine Original Articles medicine.disease Neoplastic Cells Circulating 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer cell Cancer research biology.protein Keratins Original Article Female Antibody business HeLa Cells |
| Zdroj: | Cancer Science |
| ISSN: | 1349-7006 1347-9032 |
| Popis: | Circulating tumor cells (CTC) are newly discovered biomarkers of cancers. Although many systems detect CTC, a gold standard has not yet been established. We analyzed CTC in uterine cervical cancer patients using an advanced version of conditionally replicative adenovirus targeting telomerase-positive cells, which was enabled to infect coxsackievirus-adenovirus receptor-negative cells and to reduce false-positive signals in myeloid cells. Blood samples from cervical cancer patients were hemolyzed and infected with the virus and then labeled with fluorescent anti-CD45 and anti-pan cytokeratin antibodies. GFP (+)/CD45 (-) cells were isolated and subjected to whole-genome amplification followed by polymerase chain reaction analysis of human papillomavirus (HPV) DNA. CTC were detected in 6 of 23 patients with cervical cancers (26.0%). Expression of CTC did not correlate with the stage of cancer or other clinicopathological factors. In 5 of the 6 CTC-positive cases, the same subtype of HPV DNA as that of the corresponding primary lesion was detected, indicating that the CTC originated from HPV-infected cancer cells. These CTC were all negative for cytokeratins. The CTC detected by our system were genetically confirmed. CTC derived from uterine cervical cancers had lost epithelial characteristics, indicating that epithelial marker-dependent systems do not have the capacity to detect these cells in cervical cancer patients. |
| Databáze: | OpenAIRE |
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