Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast
Autor: | Pauline Vasseur, Salih Topal, Craig L. Peterson, Marta Radman-Livaja |
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Přispěvatelé: | Centre Européen de Réalité Virtuelle (CERV), École Nationale d'Ingénieurs de Brest (ENIB) |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Transcriptional Activation
0301 basic medicine Saccharomyces cerevisiae Proteins Cell division Nucleosome assembly Science General Physics and Astronomy Saccharomyces cerevisiae DNA replication Article General Biochemistry Genetics and Molecular Biology Histones 03 medical and health sciences Histone H3 0302 clinical medicine Transcription (biology) Histone post-translational modifications Nucleosome [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:Science Transcriptionally active chromatin Multidisciplinary biology Chemistry Lysine Cell Cycle Acetylation General Chemistry Cell cycle Chromatin Assembly and Disassembly Nucleosomes Cell biology Histone Code 030104 developmental biology Histone biology.protein lcsh:Q Gene expression Transcription 030217 neurology & neurosurgery |
Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019) Nature Communications Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.4372. ⟨10.1038/s41467-019-12400-5⟩ |
ISSN: | 2041-1723 |
DOI: | 10.1038/s41467-019-12400-5⟩ |
Popis: | Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56Ac on transcription throughout the yeast cell cycle. We find that H3K56Ac is a genome-wide activator of transcription. While H3K56Ac has a major impact on transcription initiation, it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. We also detect a stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, a single histone mark can exert both positive and negative impacts on transcription that are coupled to different cell cycle events. H3K56Ac promotes nucleosome turnover at promoter-proximal locations and assembly of new nucleosomes during S phase. Here the authors find that H3K56Ac is both a genome-wide activator of transcription in yeast while also repressing promiscuous transcription that occurs after replication fork passage. |
Databáze: | OpenAIRE |
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