Distinct transcriptional roles for Histone H3-K56 acetylation during the cell cycle in Yeast

Autor: Pauline Vasseur, Salih Topal, Craig L. Peterson, Marta Radman-Livaja
Přispěvatelé: Centre Européen de Réalité Virtuelle (CERV), École Nationale d'Ingénieurs de Brest (ENIB)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Transcriptional Activation
0301 basic medicine
Saccharomyces cerevisiae Proteins
Cell division
Nucleosome assembly
Science
General Physics and Astronomy
Saccharomyces cerevisiae
DNA replication
Article
General Biochemistry
Genetics and Molecular Biology

Histones
03 medical and health sciences
Histone H3
0302 clinical medicine
Transcription (biology)
Histone post-translational modifications
Nucleosome
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology

lcsh:Science
Transcriptionally active chromatin
Multidisciplinary
biology
Chemistry
Lysine
Cell Cycle
Acetylation
General Chemistry
Cell cycle
Chromatin Assembly and Disassembly
Nucleosomes
Cell biology
Histone Code
030104 developmental biology
Histone
biology.protein
lcsh:Q
Gene expression
Transcription
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications, Nature Publishing Group, 2019, 10 (1), pp.4372. ⟨10.1038/s41467-019-12400-5⟩
ISSN: 2041-1723
DOI: 10.1038/s41467-019-12400-5⟩
Popis: Dynamic disruption and reassembly of promoter-proximal nucleosomes is a conserved hallmark of transcriptionally active chromatin. Histone H3-K56 acetylation (H3K56Ac) enhances these turnover events and promotes nucleosome assembly during S phase. Here we sequence nascent transcripts to investigate the impact of H3K56Ac on transcription throughout the yeast cell cycle. We find that H3K56Ac is a genome-wide activator of transcription. While H3K56Ac has a major impact on transcription initiation, it also appears to promote elongation and/or termination. In contrast, H3K56Ac represses promiscuous transcription that occurs immediately following replication fork passage, in this case by promoting efficient nucleosome assembly. We also detect a stepwise increase in transcription as cells transit S phase and enter G2, but this response to increased gene dosage does not require H3K56Ac. Thus, a single histone mark can exert both positive and negative impacts on transcription that are coupled to different cell cycle events.
H3K56Ac promotes nucleosome turnover at promoter-proximal locations and assembly of new nucleosomes during S phase. Here the authors find that H3K56Ac is both a genome-wide activator of transcription in yeast while also repressing promiscuous transcription that occurs after replication fork passage.
Databáze: OpenAIRE
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