Simian immunodeficiency virus (SIV)-specific CD8+ cytotoxic T lymphocyte responses of naive and vaccinated cynomolgus macaques infected with SIVmac32H(J5): quantitative analysis by in vitro antigenic stimulation
| Autor: | Ellen G.J. Hulskotte, Carel A. van Baalen, Marlinda E. M. Dings, Albert D. M. E. Osterhaus, Geert van Amerongen, Anna Maria Geretti |
|---|---|
| Přispěvatelé: | Virology |
| Rok vydání: | 1997 |
| Předmět: |
Cellular immunity
animal diseases viruses Simian Acquired Immunodeficiency Syndrome Gene Products gag Indicator Dilution Techniques chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Biology medicine.disease_cause Virus Antigen SDG 3 - Good Health and Well-being Antibody Specificity medicine Animals Cytotoxic T cell Antigens Viral General Veterinary General Immunology and Microbiology Vaccination SAIDS Vaccines Public Health Environmental and Occupational Health hemic and immune systems T lymphocyte Simian immunodeficiency virus Cytotoxicity Tests Immunologic Virology Kinetics Macaca fascicularis CTL Infectious Diseases Immunology Molecular Medicine CD8 |
| Zdroj: | Vaccine, 15(11), 1269-1275. Elsevier |
| ISSN: | 0264-410X |
| Popis: | Detailed analyses of simian immunodeficiency virus (SIV)-specific cytotoxic T lymphocyte (CTL) responses in vaccinated and infected macaques may help to clarify the role of CTL immunity in protection against lentiviruses. Here, the optimal conditions for the measurement of SIV Gag-specific CTL were investigated by bulk and limiting dilution assays of peripheral blood mononuclear cells (PBMC) from naive and vaccinated cynomolgus macaques (Macaca fascicularis) infected with SIVmac32H(J5). In vitro restimulation was generally required for CTL detection. Selective activation of CD8+ and MHC-restricted SIV Gag-specific CTL was induced by stimulation with autologous para-formaldehyde-fixed B-lymphoblastoid cell lines infected with a recombinant vaccinia virus expressing SIV Gag. Applied to limiting dilution assays, antigenic stimulation reproducibly demonstrated SIV Gag-specific CTL precursors (CTLp) in PBMC of all animals studied, including those lacking significant responses in standard bulk CTL assays. |
| Databáze: | OpenAIRE |
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