NRT2.1 C‐terminus phosphorylation prevents root high affinity nitrate uptake activity in Arabidopsis thaliana
| Autor: | Alain Gojon, Adeline Mauries, Aurore Jacquot, Laurence Lejay, Véronique Santoni, Pauline Bonillo, Zhi Li, Cécile Fizames, Valentin Chaput, Fanny Bellegarde, Sonia Hem, Edith Laugier, Antoine Martin, Pascal Tillard, Waltraud X. Schulze |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plant Systems Biology, Institute of Physiology and Biotechnology of plants, Plateforme de Spectrométrie de Masse Protéomique - Mass Spectrometry Proteomics Platform (MSPP), Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
0106 biological sciences 0301 basic medicine Arabidopsis thaliana Physiology Ammonium nitrate nitrate transporter [SDV]Life Sciences [q-bio] Anion Transport Proteins Arabidopsis Plant Science Plant Roots 01 natural sciences Serine 03 medical and health sciences chemistry.chemical_compound Nitrate Gene Expression Regulation Plant [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Post-translational regulation Plant Proteins Nitrates post-translational regulation biology Arabidopsis Proteins Chemistry phosphorylation C-terminus biology.organism_classification 030104 developmental biology Biochemistry Nitrate transport root nitrate uptake NRT2.1 Phosphorylation 010606 plant biology & botany |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2020, 228 (3), pp.1038-1054. ⟨10.1111/nph.16710⟩ |
| ISSN: | 0028-646X 1469-8137 |
| DOI: | 10.1111/nph.16710⟩ |
| Popis: | International audience; In Arabidopsis thaliana, NRT2.1 codes for a main component of the root nitrate high-affinity transport system. Previous studies revealed that post-translational regulation of NRT2.1 plays an important role in the control of root nitrate uptake and that one mechanism could correspond to NRT2.1 C-terminus processing. To further investigate this hypothesis, we produced transgenic plants with truncated forms of NRT2.1. It revealed an essential sequence for NRT2.1 activity, located between the residues 494-513. Using a phospho-proteomic approach, we found that this sequence contains one phosphorylation site, at serine 501, which can inactivate NRT2.1 function when mimicking the constitutive phosphorylation of this residue in transgenic plants. This phenotype could neither be explained by changes in abundance of NRT2.1 and NAR2.1, a partner protein of NRT2.1, nor by a lack of interaction between these two proteins. Finally, the relative level of serine 501 phosphorylation was found to be increased by ammonium nitrate in wild-type plants, leading to the inactivation of NRT2.1 and to a decrease in high affinity nitrate transport into roots. Altogether, these observations reveal a new and essential mechanism for the regulation of NRT2.1 activity. |
| Databáze: | OpenAIRE |
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