Synergistic short-term and long-term effects of TGF-β1 and 3 on collagen production in differentiating myoblasts

Autor: Rob C. I. Wüst, Michèle M. G. Hillege, Gang Wu, Richard T. Jaspers, Andi Shi
Přispěvatelé: Physiology, AMS - Musculoskeletal Health, AMS - Tissue Function & Regeneration, AMS - Ageing & Vitality, AMS - Rehabilitation & Development
Rok vydání: 2021
Předmět:
Zdroj: Biochemical and Biophysical Research Communications, 547, 176-182. Academic Press Inc.
Shi, A, Hillege, M M G, Wüst, R C I, Wu, G & Jaspers, R T 2021, ' Synergistic short-term and long-term effects of TGF-β1 and 3 on collagen production in differentiating myoblasts ', Biochemical and Biophysical Research Communications, vol. 547, pp. 176-182 . https://doi.org/10.1016/j.bbrc.2021.02.007
ISSN: 1090-2104
0006-291X
DOI: 10.1016/j.bbrc.2021.02.007
Popis: Skeletal muscle fibrosis and regeneration are modulated by transforming growth factor β (TGF-β) superfamily. Amongst them, TGF-β1 is a highly potent pro-fibrotic factor, while TGF-β3 has been implicated to reduce scar formation and collagen production in skin and vocal mucosa. However, little is known about the individual and combined short- and long-term effects of TGF-β1 and TGF-β3 on collagen expression in myoblasts and myotubes. Here we show that in C2C12 myoblasts TGF-β1 and/or TGF-β3 increased mRNA expression of Ctgf and Fgf-2 persistently after 3 h and of Col1A1 after 24 h, while TGF-β1+TGF-β3 mitigated these effects after 48 h incubation. Gene expression of Tgf-β1 was enhanced by TGF-β1 and/or TGF-β3 after 24 h and 48 h. However, Tgfbr1 mRNA expression was reduced at 48 h. After 48 h incubation with TGF-β1 and/or TGF-β3, Col3A1 and Col4A1 mRNA expression levels were decreased. Myoblasts produced collagen after three days incubation with TGF-β1 and/or TGF-β3 in a dose independent manner. Collagen deposition was doubled when myoblasts differentiated into myotubes and TGF-β1 and/or TGF-β3 did not stimulate collagen production any further. TGF-β type I receptor (TGFBR1) inhibitor, LY364947, suppressed TGF-βs-induced collagen production. Collagen I expression was higher in myotubes than in myoblasts. TGF-β1 and/or TGF-β3 inhibited myotube differentiation which was antagonized by LY364947. These results indicate that both C2C12 myoblasts and myotubes produce collagen. Whereas TGF-β1 and TGF-β3 individually and simultaneously stimulate collagen production in C2C12 differentiating myoblasts, in myotubes these effects are less prominent. In muscle cells, TGF-β3 is ineffective to antagonize TGF-β1-induced collagen production.
Databáze: OpenAIRE
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