Effects of pH and Temperature on the Composition of Polar Lipids in Thermoplasma acidophilum HO-62
| Autor: | Yasuo Shida, Tairo Oshima, Naoki Nemoto, Haruo Shimada, Akihiko Yamagishi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Thermoplasma
Physiology and Metabolism Glyceryl Ethers Microbiology Permeability chemistry.chemical_compound Glycolipid Moiety Sugar Cyclopentane Molecular Biology Chromatography High Pressure Liquid Phospholipids Liposome Chromatography Molecular Structure biology Temperature Thermoplasma acidophilum Hydrogen-Ion Concentration biology.organism_classification Caldarchaeol Biochemistry chemistry Liposomes Glycolipids Protons |
| Zdroj: | Journal of Bacteriology. 190:5404-5411 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Thermoplasma acidophilum HO-62 was grown at different pHs and temperatures, and its polar lipid compositions were determined. Although the number of cyclopentane rings in the caldarchaeol moiety increased when T. acidophilum was cultured at high temperature, the number decreased at low pHs. Glycolipids, phosphoglycolipids, and phospholipids were analyzed by high-performance liquid chromatography with an evaporative light-scattering detector. The amount of caldarchaeol with more than two sugar units on one side increased under low-pH and high-temperature conditions. The amounts of glycolipids increased and those of phosphoglycolipids decreased under these conditions. The proton permeability of the liposomes obtained from the phosphoglycolipids that contained two or more sugar units was lower than that of the liposomes obtained from the phosphoglycolipids that contained one sugar unit. From these results, we propose the hypothesis that T. acidophilum adapts to low pHs and high temperatures by extending sugar chains on their cell surfaces, as well as by varying the number of cyclopentane rings. |
| Databáze: | OpenAIRE |
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