Community structure of denitrifying and total bacteria during nitrogen accumulation in an ammonia-loaded biofilter

Autor: Kazutaka Kuroda, T. Matsumoto, Tomoko Yasuda, Yasuyuki Fukumoto, Dai Hanajima, Miyoko Waki, Kazuyoshi Suzuki, Hirohide Uenishi
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: SC30201810110009
NARO成果DBa
ISSN: 1364-5072
DOI: 10.1111/jam.13603
Popis: Aims To obtain insight into the complex behaviour of denitrifying and total bacterial groups during the nitrogen accumulation process in an ammonia-loaded biofiltration system. Methods and results Denitrifying and total bacterial communities in a laboratory-scale rockwool biofilter with intermittent water recirculation were analysed by using denaturing gradient gel electrophoresis targeting nosZ and metabarcoding sequencing of the 16S rRNA gene. Gene abundance was evaluated by quantitative PCR. The nosZ number increased from 6·59 × 106 to 3·33 × 108 copies per gram dry sample over the 436 days of operation, during which nitrogen mass balance errors increased to 39%. The nosZ sequences associated with the genera Castellaniella, Hyphomicrobium and Pseudomonas were detected. Metabarcoding sequencing analysis indicated that the proportions of the genera for which at least one denitrifying strain or species possessing nosZ had been characterized corresponded well to the nitrogen loss. In addition, the genus Nitrosococcus (γ-proteobacteria) increased its relative abundance at days 317 and 436. Conclusions The increased proportion of denitrifying bacteria in this ammonia-loaded biofiltration system could be related to the nitrogen loss. Significance and impact of the study These results will help to clarify the complex behaviour of nitrifiers and denitrifiers within ammonia-loaded biofiltration systems.
Databáze: OpenAIRE
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