Maternal Progesterone Treatment Reduces Maternal Inflammation-Induced Fetal Brain Injury in a Mouse Model of Preterm Birth
| Autor: | Ofer Fainaru, Ola Gutzeit, Michael Y. Divon, Nizar Khatib, Yuval Ginsberg, Zeev Weiner, Salim Hadad, Ron Beloosesky |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Placenta medicine.medical_treatment Anti-Inflammatory Agents medicine.disease_cause Antioxidants Andrology chemistry.chemical_compound Western blot Pregnancy medicine Animals Saline Progesterone Inflammation Mice Inbred ICR Fetus medicine.diagnostic_test business.industry Brain Obstetrics and Gynecology Administration Intravaginal Disease Models Animal Oxidative Stress Neuroprotective Agents medicine.anatomical_structure chemistry Brain Injuries Cytokines Premature Birth Gestation Female Tumor necrosis factor alpha Inflammation Mediators business Oxidative stress |
| Zdroj: | Reproductive Sciences. 28:166-176 |
| ISSN: | 1933-7205 1933-7191 |
| DOI: | 10.1007/s43032-020-00272-w |
| Popis: | Maternal natural vaginal progesterone (nVP) administration has been shown to reduce the risk of preterm birth (PTB). The largest randomized trial of nVP for PTB (OPPTIMUM) noted a sonographic reduction in neonatal brain injury following nVP treatment. We investigated the neuroinflammatory protective effect of maternal nVP in a mouse model for maternal inflammation. Pregnant mice (n = 24) were randomized to nVP (1 mg/day) or vehicle from days 13–16 of gestation. At days 15 and 16, lipopolysaccharide (30 μg) or saline were administered. Mice were sacrificed 4 h following the last injection. Fetal brains and placentas were collected. Levels of NF-κB, nNOS, IL-6, and TNFα were determined by Western blot. Maternal lipopolysaccharide significantly increased fetal brain levels of IL-6 (0.33 ± 0.02 vs. 0.11 ± 0.01 u), TNFα (0.3 ± 0.02 vs. 0.10 ± 0.01 u), NF-κB (0.32 ± 0.01 vs. 0.17 ± 0.01 u), and nNOS (0.24 ± 0.04 vs. 0.08 ± 0.01 u), and reduced the total glutathione levels (0.014 ± 0.001 vs. 0.026 ± 0.001 pmol/μl; p |
| Databáze: | OpenAIRE |
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