Strength and medium-term impact of HisAK70 immunization in dogs: Vaccine safety and biomarkers of effectiveness for ex vivo Leishmania infantum infection
| Autor: | Abel Martínez-Rodrigo, P. Arias, Javier Carrión, Javier Fernández-Cotrina, Silvia Belinchón-Lorenzo, Ricardo de la Fuente, Gustavo Domínguez-Bernal, José A. Orden, Alicia Mas |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Adoptive cell transfer 040301 veterinary sciences CpG Oligodeoxynucleotide T-Lymphocytes medicine.medical_treatment 030231 tropical medicine Immunology Heterologous Antigens Protozoan Microbiology 0403 veterinary science 03 medical and health sciences Dogs 0302 clinical medicine Adjuvants Immunologic Vaccines DNA medicine Animals Immunology and Allergy Leishmania infantum Leishmaniasis Vaccines General Veterinary biology Dendritic Cells 04 agricultural and veterinary sciences General Medicine Th1 Cells biology.organism_classification Adoptive Transfer Coculture Techniques Vaccination Disease Models Animal Infectious Diseases Immunization Leishmaniasis Visceral Female Peptides Adjuvant Biomarkers Ex vivo Plasmids |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 65:137-143 |
| ISSN: | 0147-9571 |
| DOI: | 10.1016/j.cimid.2019.05.009 |
| Popis: | HisAK70 candidates have successfully been tested in cutaneous (CL) and visceral leishmaniosis (VL) mouse models. Here, we analyse different biomarkers in dog trials after a heterologous immunization strategy with a HisAK70 candidate (plasmid DNA plus adoptive transfer of peripheral blood-derived dendritic cells (DCs) pulsed with the same pathoantigen and CpG ODN as an adjuvant) to explore the antileishmanial activity in an ex vivo canine co-culture system in the presence of Leishmania infantum parasites. In the canine model, the heterologous HisAK70 vaccine could decrease the infection index in the DC-T cell co-culture system by up to 54% after 30 days and reach almost 67% after 100 days post-immunization, respectively, compared to those obtained in the control group of dogs. The observed security and potential to fight ex vivo L. infantum infection highlight a HisAK70 heterologous immunization strategy as a promising alternative to evaluate its effectiveness against canine VL. |
| Databáze: | OpenAIRE |
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