Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
| Autor: | Benjamin L Krog, Michael D. Henry, Sophia Williams-Perez, Devon L. Moose, Renee Cafun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
General Immunology and Microbiology
Syringes General Chemical Engineering General Neuroscience Hemodynamics Neoplastic Cells Circulating medicine.disease General Biochemistry Genetics and Molecular Biology Orders of magnitude (mass) Metastasis Extracellular matrix Lymphatic system Circulating tumor cell Cancer cell Shear stress medicine Humans Biomedical engineering |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/62478 |
| Popis: | During metastasis, cancer cells from solid tissues, including epithelia, gain access to the lymphatic and hematogenous circulation where they are exposed to mechanical stress due to hemodynamic flow. One of these stresses that circulating tumor cells (CTCs) experience is fluid shear stress (FSS). While cancer cells may experience low levels of FSS within the tumor due to interstitial flow, CTCs are exposed, without extracellular matrix attachment, to much greater levels of FSS. Physiologically, FSS ranges over 3-4 orders of magnitude, with low levels present in lymphatics ( 500 dynes/cm2). There are a few in vitro models designed to model different ranges of physiological shear stress over various time frames. This paper describes a model to investigate the consequences of brief (millisecond) pulses of high-level FSS on cancer cell biology using a simple syringe and needle system. |
| Databáze: | OpenAIRE |
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