Preparation, characterization and trifluralin degradation of laccase-modified cellulose nanofibers
| Autor: | Anupama Kaushik, Dharamender Kumar, Monica Bansal, Ghanshyam S. Chauhan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Immobilized enzyme Materials Science (miscellaneous) 02 engineering and technology 01 natural sciences Nanocellulose chemistry.chemical_compound lcsh:TA401-492 Chemical Engineering (miscellaneous) lcsh:TJ163.26-163.5 Laccase Catechol 010405 organic chemistry Renewable Energy Sustainability and the Environment Trifluralin Substrate (chemistry) Biodegradation 021001 nanoscience & nanotechnology 0104 chemical sciences Fuel Technology chemistry lcsh:Energy conservation Degradation (geology) lcsh:Materials of engineering and construction. Mechanics of materials 0210 nano-technology Nuclear chemistry |
| Zdroj: | Materials Science for Energy Technologies, Vol 1, Iss 1, Pp 29-37 (2018) |
| ISSN: | 2589-2991 |
| Popis: | Laccase, an oxidoreductase enzyme, can biodegrade persistent pesticides and other xenobiotics to less toxic monomers. The present work involves immobilization of laccase onto activated nanocellulose fibers (CNFs) extracted from bagasse. The laccase grafted CNFs were characterized by different techniques. %Immobilization and the activity, stability and reusability of the immobilized laccase was studied using 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate. The %immobilization was calculated to be 85% with a maximum activity of 0.38 U/mg. 60.5% activity was retained even after 15 repeated uses of the immobilized enzyme. Moreover the immobilized enzyme was found to be stable with 75% relative activity after a period of 45 days. The laccase grafted CNFs were evaluated in degradation of trifluralin, a toxic pesticide resistant to natural transformation or degradation processes, in the presence of humic monomers, guiacol and catechol. Results show 100% degradation of trifluralin in 24 h in the presence of guiacol as mediator. Keywords: Biowaste utilization, Cellulose nanofibers, Laccase immobilization, Trifluralin degradation, Reusability and storability |
| Databáze: | OpenAIRE |
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