'Over-inlay' block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats
| Autor: | Gallia Graiani, Roberto Sala, Simone Lumetti, Francesca Ravanetti, G. Ghiacci, Guido Maria Macaluso, Edoardo Manfredi, Antonio Cacchioli, Carlo Galli |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Histology Inlay Surgical procedure business.industry Skull 030206 dentistry Bone healing Surgery Bone regeneration 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Bone transplantation Block (telecommunications) medicine Periodontics Rat Oral Surgery business Research Article Biomedical engineering |
| Zdroj: | Journal of periodontal & implant science 46 (2016): 220–233. doi:10.5051/jpis.2016.46.4.220 info:cnr-pdr/source/autori:Ghiacci, Giulia; Graiani, Gallia; Ravanetti, Francesca; Lumetti, Simone; Manfredi, Edoardo; Galli, Carlo; Cacchioli, Antonio; Macaluso, Guido Maria; Sala, Roberto/titolo:"Over-inlay" block graft and differential morphometry: a novel block graft model to study bone regeneration and host-to-graft interfaces in rats/doi:10.5051%2Fjpis.2016.46.4.220/rivista:Journal of periodontal & implant science/anno:2016/pagina_da:220/pagina_a:233/intervallo_pagine:220–233/volume:46 Journal of Periodontal & Implant Science |
| DOI: | 10.5051/jpis.2016.46.4.220 |
| Popis: | Purpose The aim of this study was to present new a model that allows the study of the bone healing process, with an emphasis on the biological behavior of different graft-to-host interfaces. A standardized “over-inlay” surgical technique combined with a differential histomorphometric analysis is presented in order to optimize the use of critical-size calvarial defects in pre-clinical testing. Methods Critical-size defects were created into the parietal bone of 8 male Wistar rats. Deproteinized bovine bone (DBBM) blocks were inserted into the defects, so that part of the block was included within the calvarial thickness and part exceeded the calvarial height (an “over-inlay” graft). All animals were sacrificed at 1 or 3 months. Histomorphometric and immunohistochemical evaluation was carried out within distinct regions of interest (ROIs): the areas adjacent to the native bone (BA), the periosteal area (PA) and the central area (CA). Results The animals healed without complications. Differential morphometry allowed the examination of the tissue composition within distinct regions: the BA presented consistent amounts of new bone formation (NB), which increased over time (24.53%±1.26% at 1 month; 37.73%±0.39% at 3 months), thus suggesting that this area makes a substantial contribution toward NB. The PA was mainly composed of fibrous tissue (71.16%±8.06% and 78.30%±2.67%, respectively), while the CA showed high amounts of DBBM at both time points (78.30%±2.67% and 74.68%±1.07%, respectively), demonstrating a slow remodeling process. Blood vessels revealed a progressive migration from the interface with native bone toward the central area of the graft. Osterix-positive cells observed at 1 month within the PA suggested that the periosteum was a source of osteoprogenitor elements. Alkaline phosphatase data on matrix deposition confirmed this observation. Conclusions The present model allowed for a standardized investigation of distinct graft-to-host interfaces both at vertically augmented and inlay-augmented sites, thus possibly limiting the number of animals required for pre-clinical investigations. Graphical Abstract |
| Databáze: | OpenAIRE |
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