Changes in cell surface anionogenic groups during differentiation ofHerpetomonas samuelpessoai mediated by dimethylsulfoxide
| Autor: | Celuta S. Alviano, Jayme Angluster, de Souza W, de Araujo Soares Rm, Costa e Silva Filho F, Esteves Mj |
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| Rok vydání: | 1988 |
| Předmět: |
Anions
Electrophoresis Iron Cellular differentiation Cell Biophysics Neuraminidase Biochemistry Cell membrane chemistry.chemical_compound Cations Electrochemistry medicine Animals Dimethyl Sulfoxide Colloids biology Dimethyl sulfoxide Cell Membrane Cell Biology General Medicine Hydrogen-Ion Concentration biology.organism_classification Sendai virus Parainfluenza Virus 1 Human Sialic acid medicine.anatomical_structure chemistry Ferritins Sialic Acids biology.protein Trypanosomatina Chromatography Thin Layer |
| Zdroj: | Cell Biophysics. 13:29-41 |
| ISSN: | 1559-0283 0163-4992 |
| DOI: | 10.1007/bf02797363 |
| Popis: | The surface anionic groups of untreated or dimethyl sulfoxide (DMSO)-treated Herpetomonas samuelpessoai cells were analyzed by cell electrophoresis, ultrastructural cytochemistry, and identification of sialic acids using thin-layer chromatography. Differentiation of H. samuelpessoai induced by DMSO treatment caused a significant increase in the net negative surface charge. In flagellates exposed to DMSO, more cationized ferritin, colloidal iron hydroxide, and sendai virus particles bound to the cell surface. Treatment of both untreated and DMSO-treated flagellates with neuraminidase decreased markedly the EPM of cells to the cathodic pole. These findings suggest that sialic acid residues are the major anionogenic groups exposed on the surface of H. samuelpessoai. Thin-layer chromatography showed that N-acetyl and N,O-diacylneuraminic acids, in equal proportions, were present in H. samuelpessoai. However, N-acetylneuraminic acid predominates in DMSO-treated cells. |
| Databáze: | OpenAIRE |
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