Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry
| Autor: | Montserrat Shelbourne, Afaf H. El-Sagheer, Tom Brown |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Azides
Oligonucleotides Alkyne 010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Labelling DNA Crosslinking Polymer chemistry Materials Chemistry Copper-free click chemistry Fluorescent Dyes chemistry.chemical_classification Bicyclic molecule 010405 organic chemistry Oligonucleotide Metals and Alloys General Chemistry 0104 chemical sciences 3. Good health Surfaces Coatings and Films Electronic Optical and Magnetic Materials Monomer chemistry Alkynes Ceramics and Composites Nucleic Acid Conformation Click Chemistry DNA Copper Thymidine |
| Zdroj: | Chemical Communications; Vol 48 Chemical Communications |
| ISSN: | 1364-548X |
| Popis: | Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes. |
| Databáze: | OpenAIRE |
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