A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis
Autor: | Jenny Carmichael, Jennifer Hirst |
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Přispěvatelé: | Hirst, Jennifer [0000-0001-9063-8494], Apollo - University of Cambridge Repository |
Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
Male
Transgene Clathrin 03 medical and health sciences 0302 clinical medicine RNA interference clathrin spermatocytes Testis Animals Drosophila Proteins lcsh:QH573-671 GGA Gene 030304 developmental biology 0303 health sciences Gene knockdown adaptor biology lcsh:Cytology fungi Cell Biology biology.organism_classification AP-1 Phenotype Molecular biology acroblast spermatogenesis Cell biology Transcription Factor AP-1 Adaptor Proteins Vesicular Transport Fertility biology.protein Female RNA Interference Drosophila Drosophila melanogaster Spermatogenesis 030217 neurology & neurosurgery Research Article spermatids |
Zdroj: | BMC Cell Biology, Vol 12, Iss 1, p 22 (2011) BMC Cell Biology |
ISSN: | 1471-2121 |
Popis: | Background GGAs (Golgi-localised, γ-ear containing, ADP ribosylation factor-binding) are a family of clathrin adaptors that sort a number of biologically important transmembrane proteins into clathrin-coated vesicles. Knockout and knockdown studies to determine GGA function are confounded by the fact that there are 3 GGA genes in mammalian cells. Thus Drosophila melanogaster is a useful model system to study tissue expression profiles and knockdown phenotypes as there is a single GGA ortholog. Results Here we have quantified protein expression in Drosophila and show that there is >3-fold higher expression of GGA in male flies relative to female flies. In female flies the majority of GGA expression is in the head. In male flies GGA is not only expressed at high levels in the head but there is a gender specific increased expression which is due to the abundant expression of GGA in the testes. Using a highly specific antibody we have localised endogenous GGA protein in testes squashes, and visualised it in somatic and germ line cells. We show that GGA is expressed during multiple stages of sperm development, and co-stains with a marker of the trans-Golgi Network. This is most striking at the acroblast of early spermatids. In spite of the high expression of GGA in testes, knocking down its expression by >95% using transgenic RNAi fly lines did not affect male fertility. Therefore spermatogenesis in the male flies appears to progress normally with Conclusion In Drosophila we have uncovered a potential role for GGA in the testes of male flies. The gender specific higher expression of GGA, its specific enrichment in testes and its localisation to developing spermatocytes and at the acroblast of spermatids supports a role for GGA function in Drosophila spermatogenesis, even though spermatogenesis still occurs when GGA expression is depleted to |
Databáze: | OpenAIRE |
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