Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection
| Autor: | Yoon-Sun Yi, Sangyeop Lee, Ha Young Lee, Sangmi Jun, Sung Ho Yun, Edmond Changkyun Park, Ju-yong Hyon, Chi-Won Choi, Seung Il Kim |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Streptococcus equi animal diseases Biology Microbiology Mass Spectrometry Extracellular Vesicles Mice 03 medical and health sciences Bacterial Proteins Antigen Streptococcal Infections parasitic diseases Animals Immunoprecipitation Horses 030304 developmental biology Strangles Antigens Bacterial Mice Inbred BALB C 0303 health sciences General Veterinary 030306 microbiology Streptococcal Vaccines Vaccination Reverse vaccinology Respiratory infection General Medicine Extracellular vesicle bacterial infections and mycoses biology.organism_classification respiratory tract diseases bacteria Female Horse Diseases Bacteria |
| Zdroj: | Veterinary Microbiology. 259:109165 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2021.109165 |
| Popis: | Streptococcus equi subspecies equi is a pathogenic bacterium that causes strangles, a highly contagious respiratory infection in horses and other equines. The limitations of current vaccines against S. equi infection warrants the development of an affordable, safe, and effective vaccine. Because gram-positive extracellular vesicles (EVs) transport various immunogenic antigens, they are attractive vaccine candidates. Here, we purified the EVs of S. equi ATCC 39506 and evaluated them as a vaccine candidate against S. equi infection in mice. As an initial step, comparative proteomic analysis was performed to characterize the functional features of the EVs. Reverse vaccinology and knowledge-based annotations were then used to screen potential vaccine candidates (PVCs) for S. equi ATCC 39506. Finally, 32 PVCs were found to be enriched in the EV fraction, suggesting the usefulness of this fraction as a vaccine. Importantly, a significantly higher survival rate after S. equi infection was detected in mice immunized with S. equi-derived EVs via the intraperitoneal route than in mice immunized with heat-killed bacteria. Of note, immunoprecipitation-mass spectrometry results validated various immunogenic antigens within the EV proteome. In conclusion, our results suggest that S. equi-derived EVs can serve as a vaccine candidate against S. equi infection. |
| Databáze: | OpenAIRE |
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