Lipopolysaccharide mediated mast cells induce IL-10 producing regulatory T cells through the ICOSL/ICOS axis
| Autor: | Gang Cai, Weiwei Zhang, Xiaomeng Nie, Beiying Wu, Qian Shen, Huaizhou Wang, Qiang Li |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide T cell Immunology Inflammation Biology Adaptive Immunity T-Lymphocytes Regulatory Inducible T-Cell Co-Stimulator Protein chemistry.chemical_compound Inducible T-Cell Co-Stimulator Ligand Mice Immune system medicine Immunology and Allergy Animals Mast Cells Cells Cultured NF-kappa B Interleukin Mast cell Acquired immune system humanities Coculture Techniques Cell biology Interleukin-10 Mice Inbred C57BL Toll-Like Receptor 4 Interleukin 10 medicine.anatomical_structure chemistry medicine.symptom |
| Zdroj: | Clinical immunology (Orlando, Fla.). 142(3) |
| ISSN: | 1521-7035 |
| Popis: | The mechanisms by which mast cells (MCs) regulate immune responses are still largely unknown. Here, we showed that MCs induced interleukin (IL)-10 producing T cells to regulate inflammatory responses. To gain insight into the molecules involved, we set up an in vitro system in which lipopolysaccharide (LPS) stimulated MCs and CD4(+) T cells were co-cultured. Induction of IL-10 producing regulatory T cells mainly relied on the inducible costimulator ligand (ICOSL)/ICOS axis. MCs stimulated with LPS for more than 6 weeks upregulated ICOSL expression, while icosl(-/-) BMMCs failed to induce IL-10 producing T cells. The LPS effect was mediated through NF-κB activation via the TLR4 signaling pathway. Ex vivo analysis of bronchoalveolar lavage fluid from mice with LPS-mediated pneumonia revealed ICOSL(+) MCs and IL-10 producing T cell induction. Additionally, adaptive transfer of ICOSL(+) BMMCs attenuated LPS-mediated inflammation in MC-deficient mice. This study provided new evidence for the regulatory role of MCs. |
| Databáze: | OpenAIRE |
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