Structural and functional heterogeneity of hemocyanin: intra- and inter-specific comparison in four species of portunid crabs (Crustacea: Portunidae)
| Autor: | Saša Raicevich, P. Di Muro, Mariano Beltramini, Fabio Pranovi, Folco Giomi, A Ferrarese |
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| Rok vydání: | 2007 |
| Předmět: |
Evolution
Liocarcinus holsatus 030310 physiology Population Subunit composition Zoology Aquatic Science Hemocyanin 03 medical and health sciences Hemolymph 14. Life underwater education Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences education.field_of_study Ecology biology Pseudohemocyanin Decapoda biology.organism_classification Crustacean Liocarcinus marmoreus Portunidae Liocarcinus depurator |
| Zdroj: | EPIC3Marine Biology, 151(4), pp. 1237-1247 |
| ISSN: | 1432-1793 0025-3162 |
| Popis: | This work is focused on hemocyanin (Hc) heterogeneity at population level and on the biochemical characterization of the different subunit patterns. Two different approaches have been used: we have characterized Hc subunit composition to describe the subunit polymorphism. Then, we have measured the Hc oxygen-binding affinity to disclose the physiological implications of such heterogeneity. In order to evaluate the intra- and inter-specific variability, different populations of Liocarcinus depurator, Liocarcinus marmoreus, Liocarcinus holsatus, Necora puber (Crustacea: Portunidae), from British Isles and Adriatic Sea, have been sampled. Results indicate that Hc polymorphism normally occurs at both intra- and inter-specific levels and involves the protein subunit type. These evidences extend to the portunid Hc the concept of molecular heterogeneity within species that have been previously reported for other decapod and amphipod crustaceans. Besides, the results support the view that subunit composition of crustacean Hcs is almost species-specific, but also that closely related species share a common pattern. Furthermore, this heterogeneity corresponds to different stability of the native oligomers quaternary structure and different oxygen affinity. The results are discussed in relation with the environmental regimes that characterize the different sampling areas. In addition to Hc, the hemolymph collected in specimens from different sampling areas contained also a non-respiratory pseudo-Hc. This paper reports for the first time the occurrence of pseudo-Hc at the level of population. |
| Databáze: | OpenAIRE |
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