Molecular fingerprint and developmental regulation of the tegmental GABAergic and glutamatergic neurons derived from the anterior hindbrain
| Autor: | Linas Mazutis, Marjo Salminen, Francesca Morello, Juha Partanen, Daniel Borshagovski, Kaia Achim, Nuri Estartús, Laura Tikker, Laura Lahti, Mantas Survila, Alessio Delogu, Petri Törönen, Samir Sadik-Ogli, Laura Knaapi, Anna Kirjavainen |
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| Přispěvatelé: | Developmental neurogenetics, Faculty of Biological and Environmental Sciences, Centre of Excellence in Stem Cell Metabolism, Molecular and Integrative Biosciences Research Programme, Institute of Biotechnology, Computational genomics, Veterinary Biosciences, Marjo Salminen / Principal Investigator, Veterinary Biochemistry and Cell Biology, Biosciences, Genetics, Doctoral Programme Brain & Mind |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Gata2 PROGENITOR single cell transcriptomics brainstem 0302 clinical medicine Neural Stem Cells TRANSCRIPTION FACTOR GABAergic Neurons rhombomere 1 T-Cell Acute Lymphocytic Leukemia Protein 1 transcription factor Receptors Notch Forkhead Box Protein O1 Neurogenesis 1184 Genetics developmental biology physiology Cell Differentiation FAMILY DNA-Binding Proteins NOTCH neurogenesis DIFFERENTIATION Zfpm2 EXCITATORY NEURONS GABAergic Female Brainstem Notch Sox14 Tal1 Signal Transduction Dorsal Raphe Nucleus EXPRESSION SOX14 GENES Tegmentum Mesencephali FATE Glutamic Acid Hindbrain Biology General Biochemistry Genetics and Molecular Biology Midbrain 03 medical and health sciences Glutamatergic Animals Cell Lineage RNA Messenger CELL Homeodomain Proteins Embryo Mammalian Mice Inbred C57BL Rhombencephalon 030104 developmental biology MRNA Sequencing SOXB2 Transcription Factors 1182 Biochemistry cell and molecular biology Neuroscience 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Morello, F, Borshagovski, D, Survila, M, Tikker, L, Sadik-Ogli, S, Kirjavainen, A, Estartús, N, Knaapi, L, Lathi, L, Törönen, P, Mazutis, L, Delogu, A, Salminen, M, Achim, K & Partanen, J 2020, ' Molecular fingerprint and developmental regulation of the tegmental GABAergic and glutamatergic neurons derived from the anterior hindbrain ', Cell Reports, vol. 33, no. 2, pp. 108268 . https://doi.org/10.1016/j.celrep.2020.108268 Cell reports, Cambridge, MA : Cell Press, 2020, vol. 33, iss. 2, art. no. 108268, p. [1-26] |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.108268 |
| Popis: | Tegmental nuclei in the ventral midbrain and anterior hindbrain control motivated behavior, mood, memory and movement. These nuclei contain inhibitory GABAergic and excitatory glutamatergic neurons, whose molecular diversity and development remain largely unraveled. Many of the tegmental neurons originate in the embryonic ventral rhombomere 1, where the GABAergic fate is regulated by the transcription factor Tal1. Here, we used single-cell mRNA sequencing of the mouse ventral rhombomere 1 to characterize the Tal1-dependent and -independent neuronal precursors. We describe gene expression dynamics during bifurcation of the GABAergic and glutamatergic lineages and show how active Notch signaling promotes GABAergic fate selection in post-mitotic precursors. We identify GABAergic precursor subtypes that give rise to distinct tegmental nuclei and demonstrate that Sox14 and Zfpm2, two transcription factors downstream of Tal1, are necessary for the differentiation of specific tegmental GABAergic neurons. Our results provide a framework for understanding the development of cellular diversity in the tegmental nuclei |
| Databáze: | OpenAIRE |
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