Chromosomal localisation of five genes in Perkinsus olseni (Phylum Perkinsozoa)
| Autor: | M. Leonor Teles-Grilo, Américo Marques, Carlos Azevedo, Joana Tato-Costa, Carlos Conde |
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| Rok vydání: | 2010 |
| Předmět: |
Gel electrophoresis
Genetics Genetic Markers Genes Protozoan Molecular Sequence Data Chromosome Chromosome Mapping Nucleic Acid Hybridization Karyotype Sequence Analysis DNA Biology DNA Protozoan biology.organism_classification Microbiology Molecular biology DNA sequencing 18S ribosomal RNA Chromosomes Electrophoresis Gel Pulsed-Field Gene mapping Alveolata Perkinsus Gene |
| Zdroj: | European journal of protistology. 48(3) |
| ISSN: | 1618-0429 |
| Popis: | The molecular karyotype of Perkinsus olseni, a pathogenic protist that infects the clam Ruditapes decussatus, comprises nine chromosomes, ranging in size from 0.15 Mb to 6.5 Mb, representing a haploid genome of about 28 Mb. In order to establish chromosome specific markers, PCR-amplified DNA sequences belonging to five conserved genes (18S rRNA, actin type I, hsp90, β-tubulin and calmodulin) were hybridised to chromosomal bands separated by pulsed-field gel electrophoresis. Three of those probes (actin type I, hsp90 and calmodulin) hybridised to only one chromosome and the remaining two (18S rRNA and β-tubulin) hybridised to two chromosomes. In the first place, the hybridisation pattern obtained serves to dispel any doubt about the nuclear location of the smallest chromosome observed in the molecular karyotype of Perkinsus olseni. Additionally, it will be a reference for further analysis of karyotype polymorphisms in the genus Perkinsus. |
| Databáze: | OpenAIRE |
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